The results showed that Sp1 overexpression inhibited the expression and promoter activity of PTEN and correspondingly upregulated AKT phosphorylation, whereas Sp1 knockdown upregulated the expression and promoter ability of PTEN and downregulated AKT phosphorylation.

2022年4月14日 · Sp1 transcription factor (SP1) was predicted and identified as an upstream regulator of PTEN. SP1 suppressed PTEN transcription. Silencing of SP1 enhanced mouse survival and alleviated the lung injury, and it promoted the M1 polarization of macrophages in murine lung tissues.

2012年10月26日 · In this study, we examined (i) whether Sp1 could inhibit the expression and promoter activity of PTEN through Sp1-binding sites on the PTEN promoter, (ii) whether Sp1 acetylation and HDAC1 recruitment are responsible for the Sp1 inhibition of PTEN and (iii) whether Sp1 is involved in the development and aggressiveness of cancers.

2012年10月15日 · PTEN regulates Sp1 via suppression of downstream kinases, which leads to hypo-phosphorylation of Sp1 and suppresses Sp1 DNA binding. One of the pathways downstream of PTEN is the PI3K pathway which is often activated in cancers and PTEN suppresses PI3K (Georgescu, 2010, Pore et al., 2004).

2009年2月13日 · Studies using PTEN mutants show that its protein phosphatase activity directly modulates p75NTR protein expression. Nuclear relocalization of PTEN subsequent to LN stimulation suggests transcriptional control of p75NTR expression, which was confirmed following EMSA and ChIP analysis of Sp1 transcription factor binding activity.

2018年6月29日 · Consistently, the abundance of phosphatase and tension homolog deleted on chromosome ten (PTEN), a negative downstream target of SP1, was increased with the ectopic miR-150-3p. Collectively, these results suggested that miR-150-3p suppressed the growth of glioma cells partially via regulating SP1 and possibly PTEN. Previous Next glioma miR-150 ...

SP1 敲低减弱了 PTEN 敲低引起的 AKT/mTOR 通路的激活。 体内结果表明，SP1 敲低可抑制肿瘤生长，增加 PTEN 表达，并降低 AKT/mTOR 通路相关蛋白水平。 结论 SP1 通过抑制 PTEN 介导的 AKT/mTOR 通路促进膀胱癌进展。 靶向 SP1 可能是治疗膀胱癌的潜在治疗策略。

2020年12月17日 · Mechanistic analyses revealed that miR-4310 promoted glioma progression through the PI3K/AKT pathway by targeting PTEN. Additionally, SP1 induced the expression of miR-4310 by binding to its promoter region.

2020年12月17日 · Mechanistic analyses revealed that miR-4310 promoted glioma progression through the PI3K/AKT pathway by targeting PTEN. Additionally, SP1 induced the expression of miR-4310 by binding to its promoter region.

From a mechanistic point of view, Sp1 can inhibit PTEN expression by binding to the PTEN promoter region (139). Down-regulation of miR-22 is associated with disease progression and metastasis...