... confirm the suppressive effect of CHX on the tran- scription-independent pathway, we analyzed its effect on the translocation of p53 to mitochondria, a key initial event in this pathway, in...

Deprivation of serum from the culture medium causes apoptosis in HepG2 cells, and CHX dramatically protects cells from death. p53, p21, and Bax protein levels were elevated, and cell cycle arrest was produced after serum withdrawal. CHX abolished this elevation of p53, p21, and Bax as well as the cell cycle arrest induced by serum deprivation.

2021年5月25日 · Here, we reported that a new quinolone compound RYL-687 significantly inhibited non-small cell lung cancer (NSCLC) cells which express wild type (wt) p53, in contract to its much weaker...

2014年6月27日 · In the present study, we performed an integrated analysis of genome-wide short hairpin RNA screen and gene expression data and uncovered a previously unrecognized role of Sp1 as a central modulator...

2021年7月19日 · TP53 is one of the most extensively studied tumor suppressor genes whose multifaceted mechanisms involve apoptosis, ferroptosis, DNA repair, genomic stabilization, and angiogenesis [2]. Thus,...

CHX blocks p53 accumulation, apoptosis, and p53-dependent transcriptional response. HCT116 cells were pretreated with or without CHX for 30 min (CHX-0.5 h) before addition of 5-FU. Cells were...

请问有伙伴做CHX看蛋白稳定性的吗？ 我们用到100mg/ml的浓度，24h变化都不明显，用p53作为阳性对照，也是24h才有少许降低，内参也跟着降低。 是在具体实验中有什么特别注意的而没有注意到吗？ 请问有伙伴做CHX看蛋白稳定性的吗？ 我们用到100mg/ml的浓度，24h变化都不明显，用p53作为阳性对照，也是24h才有少许降低，内参也跟着降低。 是在具体实验中有什么特别注意的而没有注意到吗？

We show that p53 identifies MCL tumours with TP53 missense mutations with high accuracy, and that sub‐clones of p53‐positive tumour cells can be identified when whole tissue sections are used. We further report on the prognostic impact of both TP53 mutations and p53 overexpression, emphasising the importance in identifying these high‐risk patients.

2018年9月24日 · Here we show that the binding of MDM2 to the p53 mRNA brings ATM to the p53 polysome where it phosphorylates the nascent p53 at serine 15 and prevents MDM2-mediated degradation of p53. A single synonymous mutation in p53 codon 22 (L22L) prevents the phosphorylation of the nascent p53 protein and the stabilization of p53 following genotoxic stress.

2023年10月19日 · Background: To clarify the relationship between p53 immunohistochemistry (IHC) staining and TP53 alterations (including mutations and deletions) in large B-cell lymphomas (LBCLs) and to explore the possibility of p53 IHC expression patterns as surrogate markers for TP53 alterations.