Here we utilized a comprehensive characterization of the ligand-dependent CRBN-BRD4 interaction to demonstrate that binding between proteins that have not evolved to interact is …

2018年7月16日 · Surface plasmon resonance (SPR) is used to generate binary binding affinities of PROTACs to BTK and CRBN (Fig. 3 A and B and SI Appendix, Fig. S5). Based on previous …

2023年12月12日 · To identify the minimal SALL4 binding domain that interacts with human cereblon complexed with IMiD molecules pomalidomide and CC-220, we examined the SPR …

2024年10月15日 · We present the design of CRBN midi, a construct that readily expresses from E. coli with high yields as soluble, stable protein without DDB1. We benchmark CRBN midi for …

Here, we develop an SPR-based assay to quantify the stability of PROTAC-induced ternary complexes by measuring for the first time the kinetics of their formation and dissociation in …

2023年7月13日 · SPR and ITC methods reveal that ternary complex formation can be potently influenced by both protein-protein interactions (PPIs) and protein-small molecule interactions.

2023年3月1日 · Cereblon (CRBN) is a substrate receptor of E3 ubiquitin ligase as well as the target of thalidomide and lenalidomide, plays a vital role in endogenous protein degradation. In …

2024年1月12日 · For this system, robust SPR, BLI, and ITC assays were developed that reproduced published results demonstrating the cooperativity of ternary complex formation. In …

Surface Plasmon Resonance (SPR) technique for evaluating direct binding of cereblon CULT domain to molecules

近年来，CRBN配体逐渐成为一个非常流行的E3连接酶配体，不仅可以用作E3连接酶配体来设计合成PROTACs，也可以用来设计分子胶降解剂，在PROTACs和分子胶领域中起着非常重要的 …