Learn surface plasmon resonance (SPR) (Biacore) and biomolecular interaction analysis (kinetics) with emphasis on experiments and kinetic data analysis.

2024年11月26日 · 表面等离子共振技术(Surface plasmon resonance, SPR)作为写入中国、美国、日本药典中的生物分子互作验证技术，在小分子化合物、中药单体与靶蛋白结合亲和力测定及验证中被广泛应用。

SPR，即表面等离子共振 (Surface Plasmon Resonance, SPR)，简单来说，它通过检测生物传感芯片上配位体与分析物之间的相互作用情况，进而探测物质的性质和结构。通过这一技术，我们能够实时、精准地分析样品中的分子、蛋白质、DNA 等各类有机、无机物质，为科研 ...

Analyzing binding kinetics: Which method is best for your next SPR experiment? Read on to learn about the two most common approaches for measuring binding kinetics, multi-cycle kinetics (MCK) and single-cycle kinetics (SCK), and how to select the right one for your interaction.

Learn surface plasmon resonance (SPR) (Biacore) and biomolecular interaction analysis (kinetics) with emphasis on experiments and kinetic data analysis.

Herein, we develop the first SPR-based assay to quantitatively measure the kinetics of ternary complex formation and dissociation, which we use to characterize the lifetime of ternary complexes composed of bromodomain-containing target proteins, PROTACs, and the von Hippel–Lindau E3 ligase (VHL).

Surface plasmon resonance (SPR) is one of the commonly used technologies for detailed and quantitative studies of protein-protein interactions and determination of their equilibrium and kinetic parameters. SPR provides excellent instrumentation for a label-free, real-time investigation of protein-protein interactions.

2021年4月1日 · By describing how to quantify ligand binding kinetics and interpret the data, this chapter enables investigators to evaluate the role of the temporal dimension of binding activity to their targets of interest. Therapeutic molecules exert their action by interacting with specific molecular targets.

2022年12月1日 · In this paper, we demonstrate a toolbox to kinetically characterize a biomarker measurement assay using Surface Plasmon Resonance imaging (SPRi) with ample opportunities for real time quality control by exploiting quantitative descriptions of the …

Surface plasmon resonance (SPR) can be used to analyze both binding affinities and kinetic parameters between a ligand and an analyte. SPR can be performed by either cross-linking a given ligand to a sensor chip covalently or utilizing high-affinity non-covalent interactions to secure a ligand in a particular conformation to a chip, both of ...