2023年9月21日 · Sst ChR2:Tag:SD and Sst ChR2 neurons were identified by their EYFP or mCherry signal under fluorescence illumination (LED4D, Thorlabs, coupled to a YFP or mCherry excitation filter).

2022年10月19日 · To examine whether the LHA-projecting MPL SST neurons are responsible for the observed self-grooming behavior, we injected retrogradely transported AAV expressing Cre-dependent ChR2 (retroAAV-DIO-ChR2-mCherry) into the LHA of SST-Cre mice, resulting in the specific expression of ChR2-mCherry in the LHA-projecting MPL SST neurons (Figure S1 M ...

Optical stimulation of ChR2-expressing PV (ChR2-PV) and ChR2-expressing SST (ChR2-SST) interneurons increased their firing rates (Fig. 2, C and D, left), which, in turn, decreased the firing rates of some putative excitatory neurons (Fig. 2, C …

2021年4月6日 · 光遗传最经典的蛋白是channelrhodopsins (ChR2)，是一种光敏感的阳离子通道，只要收到光的照射（最敏感的是大概470nm的蓝光），立马会打开然后允许Na+ K+等阳离子跨膜流动，从而让神经元去极化（即激活）。

2019年12月16日 · We demonstrate that SST interneurons exhibit properties indicative of a memory storage substrate, including (1) learning-dependent potentiation of synaptic transmission, (2) cue-specific...

2020年9月2日 · To achieve subtype-specific control of inhibitory interneurons, we selectively activated either PV or SST interneurons by expressing the light-activated cation channel channelrhodopsin-2 (ChR2)...

2020年7月22日 · For optical tagging of PV- or SST-expressing INs, we delivered Cre-inducible adeno-associated virus (AAV) carrying the gene for channelrhodopsin-2 (ChR2-mCherry) into the mPFC of PV-Cre (20) or SST-Cre (21) knock-in mice, respectively.

2016年8月3日 · Normal breathing in rodents requires activity of glutamatergic Dbx1-derived (Dbx1 +) preBötzinger Complex (preBötC) neurons expressing somatostatin (SST). We combined in vivo optogenetic and pharmacological perturbations to elucidate …

... investigate whether SST interneuron activation in the VCx evokes a Ca 2+ response in astrocytes, we used an AAV-based strategy ( Figure 1 A-C) to induce the expression of the light-gated...

2022年10月19日 · Here, we found that somatostatin (SST)-positive neurons in the rostral ZI of mice were activated by a visual innate fear stimulus. Optogenetic inhibition of SST-positive neurons in the rostral ZI resulted in reduced flight responses to an overhead looming stimulus.