2019年11月22日 · Crystal structure of the SVS_A2 protein (205-DREMH-209 /205-AQDLE-209 mutant) from ancestral sequence reconstruction at 2.38 A resolution

2023年7月6日 · Since the length of CotB2 (307 aa) is significantly shorter than most identified type I DTSs (340–390 aa) and the structural information for the holo form of SvS-A2 is unavailable, we propose ...

Intriguingly, engineering of SvS-A2 resulted in several highly specific variants such as variant A224I, which is an FPP-specific variant with almost 100% hedycaryol formation, while variant W156Y is a GGPP-specific variant with almost 100% spiroviolene formation.

2019年11月25日 · Crystal structure of the SVS_A2 protein (W79F,G83L mutant) from ancestral sequence reconstruction at 2.27 A resolution

In this study, we have obtained a crystal structure of a stable and soluble reconstructed ancestor of SvS and used an engineered crystallized variant thereof as a template to derive a high-confidence homology model of extant SvS.

Ancestral mutations (i.e., positions that differ between SvS-A2 and SvS-WT) are shown as sticks; yellow, exchanges with residues that have similar properties (e.g., Asp/Glu, Lys/Arg); orange,...

Semantic Scholar extracted view of "Crystal structure of the SVS_A2 protein (205-DREMH-209 /205-AQDLE-209 mutant) from ancestral sequence reconstruction at 2.38 A resolution" by R. Rudraraju et al.

6TIV: Crystal structure of the SVS_A2 protein (205-DREMH-209 /205-AQDLE-209 mutant) from ancestral sequence reconstruction at 2.38 A resolution

" MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42 (Database issue):D297-303.

Download scientific diagram | Kinetic analysis of SvS-A2 and SvS-WT. (a) In vitro product formation by ancestral and extant SvS (2 μM) using single substrate (60 μM of FPP or GGPP, striped bars ...