Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining .

The Gram stain uses four stains/reagents: crystal violet, Gram's iodine, ethanol, and safranin. Crystal violet (the primary stain ), enters the peptidoglycan of all bacteria giving them a purple color.

The safranin staining is the most widely used staining technique for cell differentiation, cell-based assays, and stem cell culture. The safranin stain is commonly used to quantify and identify the acidic proteoglycan and glycosaminoglycan in the cartilage tissues.

Safranin is a biological stain used in both cytology and histology, mostly as a counterstain to color cell nuclei red. Safranines are diazonium compounds with a distinctive green metallic luster, and they are strong bases that dye red or violet.

Safranin is a lipophilic cationic dye which accumulates in mitochondria according to the inside negative potential in energized mitochondria. In the course of this process, safranin undergoes a significant change in absorption and self-quenching of its fluorescence (Åkerman & Wikström, 1976; Perevoshchikova, Sorochkina, Zorov, & Antonenko, 2009).

2022年8月10日 · Then when again stained with safranin, they take the stain and appears red in color. Reagents Used in Gram Staining. Crystal Violet, the primary stain; Iodine, the mordant; A decolorizer made of acetone and alcohol (95%) Safranin, the counterstain; Procedure of Gram Staining. Take a clean, grease free slide.

The meaning of SAFRANINE is any of various usually red synthetic dyes that are amino derivatives of bases.

Our study found safranin to be 100% effective against Gram-positive bacteria (S. aureus), but it had only 20% efficacy against Gram-negative bacteria (Pseudomonas A). Only a few studies have looked at safranin’s antibacterial activity against microorganisms.

Safranin O (C.I. 50240) for microscopy Certistain ®, is a dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in …

Safranin is a red dye commonly used in plant microscopy to stain cell structures, making them more visible under a microscope. This dye helps highlight specific components such as nuclei and cell walls, which is crucial for understanding plant histology and tissue organization.