A sugarcane mosaic virus vector for rapid - Wiley Online Library
2021年3月24日 · An empty vector control virus (SCMV-EV) and SCMV-GFP were used to inoculate one-week-old maize seedlings. Both SCMV-GFP and SCMV-EV caused mosaic …
Dissecting the Molecular Mechanism of the Subcellular ... - Nature
2017年8月29日 · To determine the subcellular localization of SCMV P3N-PIPO, P3N-PIPO-green fluorescent protein (GFP) was expressed in Nicotiana benthamiana leaves by agroinfiltration. …
Identification of differentially regulated maize proteins conditioning ...
2017年6月19日 · Sugarcane mosaic virus (SCMV) is the most important cause of maize dwarf mosaic disease. To identify maize genes responsive to SCMV infection and that may be …
Maize splicing-mediated mRNA surveillance impeded by ... - Science
2024年8月23日 · Agrobacterium cultures harboring enhanced green fluorescent protein (eGFP), VPg-eGFP, eGFP-NIa-Pro, or SCMV-GFP were separately infiltrated into leaves of the …
Identification of maize genes conditioning the early systemic …
2025年3月4日 · We identified five cell types and found that mesophyll-4 (MS4) cells had the highest viral accumulation levels in most cells. The early systemic infection of SCMV resulted …
A sugarcane mosaic virus vector for gene expression in maize
2019年8月8日 · Here, we describe the development of a sugarcane mosaic virus (SCMV) vector for the purpose of protein expression in maize. An infectious SCMV cDNA clone was …
用于玉米基因表达的甘蔗花叶病毒载体。,Plant Direct - X-MOL
构建了感染性 SCMV cDNA 克隆，并将异源遗传元件放置在 SCMV 基因组中的蛋白 1 (P1) 和辅助成分蛋白酶 (HC-Pro) 顺反子之间。将表达绿色荧光蛋白 (GFP)、β-葡萄糖醛酸酶 (GUS) 或双 …
Development and Evaluation of Stable Sugarcane Mosaic Virus …
2021年12月16日 · At 10 dpi, wild-type SCMV with the gfp reporter gene (SCMV-GFP) caused severe mosaic symptoms and induced strong GFP fluorescence under UV light in the upper …
项目文章Cell Reports （IF 8.8）| 中国农业大学利用转录组+代谢组 …
2023年7月5日 · 甘蔗花叶病毒（sugarcane mosaic virus，SCMV）引起玉米矮化花叶病，是影响全球玉米的最具破坏性疾病之一。 然而，病毒如何诱导花叶症状的出现以及相关过程中的关 …
maize triacylglycerol lipase inhibits sugarcane mosaic virus …
2022年3月16日 · Crude extracts from maize leaves infected with SCMV with gfp reporter gene (SCMV-GFP), ground in 20-mM phosphate-buffered saline (pH 7.2) at a 1:3 w/v ratio, were …