Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the ...

2024年9月1日 · Fluorescence-based SELEX is a pivotal advancement in the field of aptamer selection, offering significant improvements in sensitivity and specificity compared to traditional SELEX methods.

2010年6月3日 · This protocol, cell-SELEX (systematic evolution of ligands by exponential enrichment), is a method that can generate DNA aptamers that can bind specifically to a cell type of interest.

2015年11月1日 · These SELEX-derived single stranded DNA and RNA molecules, called aptamers, were selected against various targets, such as proteins, cells, microorganisms, chemical compounds etc. They have a great potential in the use as novel antibodies, in cancer theragnostics and in biomedical research.

Avoiding PCR bias can greatly improve the quality of the aptamer pool during SELEX. Strategies to minimize PCR bias include limiting the amplification cycles, monitoring the amount of template, increasing primer concentrations, skipping the elongation step, and/or utilizing emulsion PCR. ssDNA regeneration

2023年7月20日 · SELEX enabled isolation of high affinity-binding oligonucleotides from a large population of random sequences, expanding the known functions of DNA and RNA into unexpected roles. Aptamers offer...

Cell-SELEX, also known as cell-based systematic evolution of ligands by exponential enrichment, is a technique derived from the SELEX method. It enables the selection of aptamers against cell surface molecules, receptors, or whole cells.

SELEX involves numerous singular processes, each of which contributes to the success or failure of aptamer generation. In this review, critical steps during aptamer selection are discussed in-depth, and specific problems are presented along with potential solutions.

2017年10月14日 · The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, …

2024年1月1日 · To facilitate researchers to understand aptamer screening development more timely, this review has updated the recent advances of SELEX methods in terms of optimizing the separation steps coupled with frontier carrier materials (e.g. MIPs, Ni-NTA beads), improving screening accuracy and efficiency integrated with some improved techniques (e.g ...