Semi-intact cells are cells that have undergone plasma membrane permeabilization by the cholesterol-dependent pore-forming cytolysin, streptolysin O (SLO). Permeabilization enables the introduction of various molecules into the cells, as well as the substitution of the original cytosol with an exogenously made cytosol prepared from cells in ...

We introduce a new method that removes portions of the plasma membrane of eukaryotic cells to form semi-intact cells. During preparation, these cells lose their soluble cytoplasmic contents, but retain secretory organelles such as the ER and Golgi complex in an intact form.

1987年8月14日 · We introduce a new method that removes portions of the plasma membrane of eukaryotic cells to form semi-intact cells. During preparation, these cells lose their soluble cytoplasmic contents, but retain secretory organelles such as the ER and Golgi complex in …

2019年8月22日 · Semi-intact cells are cells that have undergone plasma membrane permeabilization by the cholesterol-dependent pore-forming cytolysin, streptolysin O (SLO). Permeabilization enables the introduction of various molecules into the cells, as well as the substitution of the original cytosol with an exogenously made cytosol prepared from cells in ...

The three-layered cell wall is impregnated with openings or pores and pectin spicules; irregular desmid movement is caused by the flow of a gelatinous substance through these pores. Conjugation (temporary union for the exchange of nuclear material) is the…

Semi-Permeable Capsules have an aqueous core and sieve-like walls that keep the cells and nucleic acids partitioned inside, while allowing the free flow of the surrounding solution, be it molecular biology reagents, buffers or media.

Semi-intact cells may provide a useful model system for reconstitution of a broad range of cellular pro- cesses associated with the cytoplasmic domains of eu- remarkable capacity of these organelles to remain func- tionally intact after extensive cell homogenization (Fries and Rothman, 1980, 1981; Balch et al., 1984). In contrast, karyotic cells.

To enable a mechanistic analysis of soluble protein entry into cilia, we developed a method for semi-permeabilization of mammalian cells in which the plasma membrane is permeabilized while the ciliary membrane remains intact.

2000年1月15日 · Sorting of proteins from their site of synthesis to their final destination is now known to be a key cellular process, which, if impaired, can give rise to lethal defects. Gunther Blobel and his group established an experimental approach that is now used by numerous researchers to study secretory pathways.

2014年7月30日 · Semi-intact cells are cells whose plasma membrane is permeabilized with toxins by creating pores. Semi-intact cells allow for the exchange of the original cytosolic proteins with an exogenously-made cytosol prepared from cells of varying states, such as cells at different stages of the cell cycle, differentiation, or cells in a diseased state.