近年来,SESI-HRMS呼气代谢组学研究取得突破性进展 [1],首次在无损、直接分析条件下,成功获得个体呼气代谢表型 (>500种代谢物) [2],并在实验室研究阶段成功用于: (1)诊断慢性阻塞性肺病 [3]; (2)识别乳癌生物标志物 [4]; (3)表征个体生物钟 [5],为个体的疾病预警、治疗 ...

2025年3月5日 · Breath metabolomics enables noninvasive and rapid acquisition of metabolic information by detecting volatile organic compounds (VOCs) in exhaled breath. Secondary electrospray ionization high-resolution tandem mass spectrometry (SESI-HRMS/MS) offers the highest coverage for detecting breath metabolites among current real-time breath analysis techniques. Although it has been generally ...

2024年10月11日 · SESI-HRMS shows higher sensitivity for some compounds, needing GC–MS and LC-MS annotation. Optimizing fragmentation techniques is crucial for comprehensive analysis in this field. Secondary electrospray ionization coupled to high-resolution mass spectrometry (SESI-HRMS) is a powerful method for the analysis of exhaled breath in real time.

This study refines the application of the non-invasive Secondary Electrospray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) methodology for real-time and early detection of human respiratory bacterial pathogens in the respiratory tract of a mouse infection model

2022年1月2日 · Coupling a SESI source to a high-resolution mass spectrometry (HRMS), especially orbitrap instruments, enables the ability to detect volatile metabolites in real-time with resolving powers of m/Δm up to 280,000 [20].

2024年6月27日 · Breath analysis with secondary electrospray ionization (SESI) coupled to mass spectrometry (MS) is a sensitive method for breath metabolomics. To enable quantitative assessments using SESI-MS, a system was developed to introduce controlled amounts of gases into breath samples and carry out standard addition experiments.

基本原理二次电喷雾电离（SESI）与高分辨率质谱联用是发现呼出气中生物标志物的有力工具。 目前使用由甲酸水溶液 (FA) 组成的初级电喷雾来去除呼吸中的挥发性有机化合物。

2019年12月16日 · Coupled with a regular Orbitrap mass spectrometer, Super SESI opens the possibility for a whole new type of studies. Molecules as large as 700 Da can be detected in real-time in breath and air. In this mass range, and with the high-resolution mass spectrometry, the molecular formula can be easily inferred, easing the interpretation of the results.

2016年6月15日 · 呼气分析具有无损、灵敏、实时、快速等技术优势。 近年来，呼气代谢组学研宄取得突破性进展，首次在无损、直接分析条件下，成功获得个体呼气代谢表型（种代谢物）并在实验室研宄阶段成功用于...

本研究使用了一种名为 SuperSESI 的高级二次电喷雾电离 (SESI) 源，并直接连接到 Thermo Q-Exactive 高分辨率质谱仪 (HRMS)。 我们进行了一系列实验，以确定我们优化的 SESI-HRMS 平台是否可以通过体外取样来区分癌症类型volatilome 配置文件。