Set up PCR mixtures in a laminar flow cabinet equipped with an UV lamp. Wear fresh gloves for DNA purification and reaction set up. Use reagent containers, dedicated for PCR. Use positive displacement pipettes, or pipette tips with PCR set up. Use PCR-certified reagents, including high quality water (e.g., Water, nuclease-free, #R0581).

测定病毒 空斑形成单位 （PFU，plaque forming unit），用于流感病毒滴度测定及标定病毒的感染能力。 二、生物安全要求. 本实验采用 H1和H3亚型流感病毒 ，相关操作需要在 BSL-2级实验室 中进行。 三、实验材料

2024年1月21日 · pfu_sdb_entry_timeout_cnt_full：表示超时计数器已满。 pfu_sdb_entry_evict_set：在条目有效、未就绪、管线指令有效、超时计数器满，且未命中PC时，设置逐出信号。 pfu_sdb_entry_evict_clr：在预取指令有效时，清除逐出信号。 生成弹出信号（Generate Pop Signal）：

该产品是从高温嗜热菌Pyrococcus furiosis分离出的高保真耐高温DNA polymerase，分子量为90 kDa，具有3’→5’外切核酸酶活性，无5’→3’外切核酸酶活性，其扩增产物为平末端，无突出的A。 DNA聚合时延伸速度为600 bp/min；PCR产物为平末端。 其他高温DNA polymerase，如：Vent、DeepVent、Tli、UlTma等具有校正功能，但是Pfu是目前已发现的所有DNA polymerase中扩增DNA时出错率很低的。 常规 PCR、高保真 PCR、基因定点突变、 克隆表达。 每个包装包 …

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer ...

Pfu DNA polymerase, a proofreading DNA polymerase isolated from Pyrococcus furiosus, is an ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).1–3 These applications include cloning, gene …

Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available.

Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium.

Set up PCR mixtures in a laminar flow cabinet equipped with an UV lamp. Wear fresh gloves for DNA purification and reaction set up. Use reagent containers, dedicated for PCR. Use positive displacement pipettes, or pipette tips with aerosol filters …

2024年1月22日 · ct_lsu_pfu_gsdb 模块在 CPU 的预取机制中扮演着关键角色，特别是在检测有效预取的步幅模式方面。 它可能与加载操作和全局预取缓冲区交互，以根据检测到的步幅和指令提交动态调整预取策略。 提交阶段信号的涉及表明，这个模块可能会根据某些指令的完成情况更新其状态，这在高级预取策略中是常见的技术。 GET_STRIDE = 4'b1001, .