As an alternative means of producing metabolites normally produced in leaves of plants, the shooty teratomas incited by the tumor-forming Ti plasmid or a shooty mutant of Agrobacterium tumefaciens have been used for the de novo biosynthesis and biotransformation of some specific secondary products.

2022年2月19日 · The shooty teratomas are formed by transformation of the plant’s genetic materials with Agrobacterium tumefaciens nopaline or integration of A. tumefaciens Ti plasmid into the plant genome.

1991年7月29日 · Transgenic hairy roots and shooty teratomas of Atropa belladonna L., Nicotiana tabacum L. and Solanum tuberosum L. were obtained with Agrobacterium Ti and Ri plasmids. The hairy roots of A. belladonna, N. tabacum and S. tuberosum accumulated tropane alkaloids, nicotine alkaloids and steroidal alkaloids, respectively.

1996年2月12日 · Shooty teratomas of Atropa belladonna and Solanum aviculare and shooty teratomas and crown galls of a Duboisia leichhardtii X D. myoporoides hybrid were established by infecting plants with nopaline and genetically modified strains of Agrobacterium tumefaciens. Transformation was confirmed by nopaline assay and Southern blotting.

2001年1月1日 · T-DNA is excised from the Ti plasmid by endonuclease VirD2, with facilitation by VirD1 and VirC1. VirD2 nicks the bottom strand of the right border sequence after the third base and attaches to the 5′ end at the nick, forming the “leading” end of …

The tobacco alkaloids, nicotine, nornicotine and anatabine, were produced in hairy roots and in rooty teratomas but not in shooty teratomas. However, the shooty teratomas have the ability to accumulate the alkaloids and to biotransform nicotine to nornicotine.

Immature 'Redhaven' peach [Prunus persica (L.) Batsch] embryos were infected with a shooty mutant strain of Agrobacterium tumefaciens, tms328::Tn5, which carries an octopine-type Ti plasmid with a functional cytokinin gene and a mutated auxin gene.

tumefaciens strain A136, obtained from Andrew Binns (Uni- versity of Pennsylvania, Philadelphia, PA), is a derivative of strain C58 cured of its nopaline Ti plasmid (17). A348 is strain A136 carrying the octopine Ti plasmid pTiA6, and strain tms328::TnS is a shooty mutant of A348 resulting from a TrOS.

Ti plasmids are used as gene vectors for delivering useful foreign genes into target plant cells and tissues. The foreign gene is cloned in the T-DNA region of Ti-plasmid in place of unwanted sequences.

Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin.