SIFE was performed using Agarose gel-based electrophoresis kit by Helena Biosciences (SAS-1 IFE-4 kit) that utilizes monospecific antisera for immunoglobulin (Ig) G, IgM, and IgA heavy chains and κ and λ light chains.

Positive (i.e., M-Protein present), a serum Immunofixation (sIFE) test is automatically ordered and run (at additional charge). This reflexed test serves to identify the M-protein's heavy and light chain components (e.g., "IgG Kappa"). This finding …

2015年1月15日 · Serum and urine protein electrophoresis (SPEP, UPEP) and immunofixation electrophoresis (SIFE, IUFE) are commonly used in clinical laboratories for the detection and identification of M-proteins.

The SIFE protocol recommended by the manufacturer includes tenfold dilution of serum for immunofixation for gamma and kappa chains and fivefold dilution for alpha, mu and lambda chains. These recommendations were considered in …

Gel-based methods employ serum protein electrophoresis (SPEP) and immunofixation protein electrophoresis (SIFE) and are standard laboratory tests at most medical centers. Capillary zonal electrophoresis (CE) and immunosubtraction electrophoresis (ISUB) are equivalent techniques using a more automated capillary fluidic electrophoresis method.

2020年11月5日 · Diagnostic methods for monoclonal gammopathy are serum protein electrophoresis (SPEP) and serum protein immunofixation electrophoresis (SIFE). The corresponding methods for urine are urine protein electrophoresis (UPEP) and urine protein immunofixation electrophoresis (UIFE).

Screening immunofixation electrophoresis (sIFE) is a modified immunofixation (IFE) procedure that employs a single application of combined antisera directed...

2015年11月17日 · Screening immunofixation electrophoresis (sIFE) employs a single application of antisera directed against heavy and light chains that facilitates the...

protein 6 band Agarose gel by Helena Biosciences platform using SAS-1 SP-24 SB kit. SIFE was performed using Agarose gel-based electrophoresis kit by Helena Biosciences (SAS-1 IFE-4 kit) that utilizes monospecific antisera for immuno-globulin (Ig) G, IgM, and IgA heavy chains and κ and λ light chains. SFLC estimation was carried out by ...

2022年1月1日 · Testing for IgD and IgE M-proteins is often performed as a second step since these M-proteins are rare. sIFE is a gel-based technique. Serum is applied to multiple lanes of an agarose gel and proteins are initially separated using electrophoresis.