Explain what a SIM deep is and tell the tests that can be conducted in a SIM deep. List possible metabolic reasons why a species of bacteria could be producing hydrogen sulfide. Tell what the indole test examines and what enzyme it tests for.

DeepSIM performs optimally with the standard staining markers for confocal microscopy. It is the only SIM technology covering the full spectrum from 405 nm to NIR 750 nm excitation wavelength. DeepSIM offers the maximum flexibility in fluorophore choice and reduces the spectral overlap in multicolor experiments.

Using Sulfide-Indole-Motility (SIM) media, various Gram-negative enteric bacilli can be distinguished based upon their ability to release sulfide gas (H2S), produce indole from tryptophan, and move into the medium via the use of flagella. SIM medium contains ferrous ammonium sulfate and sodium thiosulfate.

2023年2月16日 · Materials . 24 hour cultures of Escherichia coli, Proteus vulgaris, and Staphylococcus aureus (enough for groups to share); SIM deeps (3 per group) test tube racks (1 per group) inoculating needles (1 per group) strikers (1 per group) Bunsen burners (1 per group)

2022年10月6日 · In this study, we developed rationalized deep learning (rDL) for structured illumination microscopy and lattice light sheet microscopy (LLSM) by incorporating prior knowledge of illumination...

SIM tubes test for 3 things: (1) hydrogen sulfide production (2) indole production, and (3) motility. Indole production: If an organism produces the enzyme tryptophanase, it will degrade tryptophan into indole, pyruvate, and ammonia. To test for the presence of tryptophanase, the agar deep is inoculated with a sterile needle and incubated for ...

由于快速、低光毒性、高染料兼容性的优势， 结构光照明显微成像 （sim）成为细胞器动态观测最有力的超分辨显微技术。

2023年7月12日 · Structured illumination microscopy (SIM) has become the standard for next-generation wide-field microscopy, offering ultrahigh imaging speed, superresolution, a...

2022年1月9日 · SIM stands for sulfide, Indole, and Motility and SIM medium is applied for the differentiation of gram-negative enteric bacilli. SIM test assists to isolate the bacteria on the basis of sulfide production, indole formation, and motility and thus SIM test is a combination of three tests in a single test tube.

DeepSim’s easy-to-use physics simulator boosts engineering productivity by delivering rapid design insights and offering unmatched scalability. By automating away complex, labor-intensive simulation setup procedures, our platform allows engineers to focus on higher-level, more impactful design decisions.