This book aims to guide both beginners and experienced professionals in best practices of single-cell sequencing analysis. It provides a comprehensive overview of essential analytical steps, from preprocessing to visualization and statistical evaluation and beyond.

Skillful sample preparation that yields a high-quality single cell or nuclei suspension will be a key determining factor for a successful experiment. That’s why we’d like to do everything we can to ensure you have the tips and know-how you need to master single cell sample preparation.

2023年6月6日 · We highlight the impact that single-cell multi-omics technologies have had in cell lineage tracing, tissue-specific and cell-specific atlas production, tumour immunology and cancer...

2024年12月13日 · Our overview of current practices and associated recommendations will improve the quality of atlases to come, facilitating the shift to a unified, reference-based understanding of single-cell...

Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-...

2025年3月18日 · Central nervous system malignancies include primary tumors and brain metastases (BrMs) originating from extracranial cancers. Bejarano et al. examine primary and metastatic brain tumors and find distinct vascular alterations in IDH mutant gliomas, IDH WT glioblastomas and BrMs, and associated differences in immune cell infiltration, providing a resource torward further understanding brain ...

Editors choose one cover from the submissions for each issue, based on both the aesthetic quality of the image and how well it represents the concepts in the study. Please note that we occasionally have issues where we do not consider cover submissions.

2013年7月9日 · We develop an enzyme-triggered permeable DNA hydrogel cover to envelop and release single cells in microwells. The porous structure of the DNA hydrogel allows nutrients and waste to pass through, leading to a cell viability as high as 98%.

2022年5月12日 · On the cover: Stereo-seq dissects cell-type composition in an E16.5 mouse embryo sagittal section. In this issue, Chen et al. combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics sequencing (Stereo-seq), which was applied to generate the Mouse Organogenesis Spatiotemporal ...

If RNA is to be isolated for downstream application, you can stain cells by using CellCover as antibody diluent and washing buffer. Note: Attachment of cells to the substrate is critical.