A single-molecule experiment is an experiment that investigates the properties of individual molecules. Single-molecule studies may be contrasted with measurements on an ensemble or bulk collection of molecules, where the individual behavior of molecules cannot be distinguished, and only average characteristics can be

2022年1月1日 · Here, we present single-molecule and accompanying ensemble methods to quantify these processes more comprehensively. In particular, we focus on how to properly design and execute a single-molecule nucleation assay, in which we detect signals arising from individual units of fluorescently labeled RNA-binding proteins associating with an RNA ...

2023年11月13日 · Our single-molecule FRET studies for freely diffusing molecules revealed that intrinsically disordered FUS-LC monomer exists as a heterogeneous ensemble of structures comprising chiefly two...

2016年2月15日 · Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules...

2021年5月4日 · We introduce TrackIt, an integrative tracking and analysis software for fast and extensive analysis of single molecule data sets within a single framework. Our user-friendly graphical user...

2007年7月31日 · We report fluorescence assays for a functionally important conformational change in bacteriophage T7 DNA polymerase (T7 pol) that use the environmental sensitivity of a Cy3 dye attached to a DNA substrate.

2024年10月1日 · Single-molecule correlated chemical probing (smCCP) strategies are now addressing this limitation by enabling detection of multiple per-nucleotide events in the same RNA strand, offering new views of RNA ensembles in their native cellular contexts.

2023年1月3日 · This chapter presents the principles of single-molecule FRET spectroscopy and the added information it gives compared to ensemble FRET spectroscopy. We describe different experimental implementations, primarily focusing on intensity-based approaches.

2021年3月29日 · Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation.

2005年9月9日 · Here, we consider the conformational space sampled and the average structural properties of CI2 in 100 independent 20-ns unfolding simulations at 498 K to determine the number of simulations needed to describe the unfolding process and to compare single-molecule and ensemble behavior.