2020年9月11日 · L929 cells stimulated with the death ligands—TNF or poly(I:C) to activate TNFR and TRIF signaling, respectively—in combination with the pan-caspase inhibitor zVAD underwent necroptosis as ...

2010年11月5日 · In this study, we tested this idea and identified cIAP1 and TAK1 as new regulators of TNF-induced programmed necrosis. A widely used cellular system for studying necrotic cell death is the mouse...

Hyperosmotic stress and SMAC mimetics synergistically kill L929 cells. (a–c) L929 cells were treated with the indicated concentrations of the SMAC mimetics (a) BV6, (b) LCL161 and (c) birinapant (Biri) in the presence and absence of 75 mM NaCl.

2019年10月23日 · Our study indicates that memTNF accumulation can be monitored to track patient responsiveness and for optimizing both the clinical dosages of MTA drugs and their combination with other...

2009年6月12日 · Similar to HT-29 cells, necrotic cell death in L929 cells was also accelerated by Smac mimetic in a concentration dependent manner (Figure S3C). Since cells resistant to TNF-α induced necrotic death are also defective in RIP3 expression, we then tested whether ectopic expression of RIP3 converts these cells to sensitive ones.

2017年4月4日 · Calcium flux in necroptosis was observed at a later stage upon treatment with the binary mixture TZ excluding a Smac mimetic. Under these conditions, L929 and NIH 3T3 cell lines underwent necroptosis but with slower kinetics (Figures 3 E and 3F).

Here, we demonstrate for the first time that Smac mimetic primes apoptosis-resistant, FADD- or caspase-8-deficient leukemia cells for TNFα-induced necroptosis in a synergistic manner. In contrast to TNFα, Smac mimetic significantly enhances CD95-induced apoptosis in wild-type but not in FADD-deficient cells.

mouse fibroblast L929 cells (in which TNF-induced necrosis was first observed), cells from the hematopoietic system, and epithelial cells of the gastrointestinal tract. During the necrosis induction process, RIP3 binds to the kinase RIP1 (which is also calledRIPK1)throughtheirrespectiveRIPhomotypicinteraction motif (RHIM) domains.

L929 fibroblastoma cells (L929-A) and L929 fibrosarcoma cells (L929-N) are different cell lines that are commonly used to study the cytotoxicity of tumor necrosis factor alpha (TNFα). TNFα has been reported to induce necrosis in both of these cell lines.

2024年2月8日 · To identify novel necroptotic inhibitors, we evaluated compounds in an in-house library for their ability to inhibit necroptosis induced by TNF-α, the Smac mimetic SM-164, and the pan-caspase...