单分子荧光原位杂交（smFISH）是分子生物学中用于检测RNA分子在细胞中定位和表达的技术。 它基于原位杂交技术，使用荧光标记的探针与目标RNA结合，形成荧光信号，通过显微镜观察可以观察到RNA的位置和数量。

RNA imaging with smFISH enables quantitative and qualitative gene expression experiments within single cells. With smFISH, researchers can measure cellular mRNA and perform spatial localization and quantification in locations such as the cytoplasm and nucleus.

2022年10月24日 · Here, we present high-throughput smFISH (HT-smFISH), a simple and cost-efficient method for imaging hundreds to thousands of single endogenous RNA molecules in 96-well plates. HT-smFISH uses...

2018年5月25日 · Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules. Complementary to deep sequencing-based methods, smFISH provides information about the cell-to-cell variation i …

Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules. Complementary to deep sequencing-based methods, smFISH ...

2023年11月15日 · 单分子荧光原位杂交（single-molecule fluorescence in situ hybridization，smFISH）技术是一种通过用偶联荧光基团的寡核苷酸探针，对固定细胞或组织中单个mRNA分子进行成像的方法。smFISH可对RNA进行定位、定量，以此对目标转录本进行实时研 …

In this chapter, we describe in detail how to perform a successful smFISH experiment and how to quantify mRNA transcripts in bacterial cells. The flexibility of the method allows for straightforward adaptation to different bacterial species and experimental...

2013年11月8日 · 单分子荧光原位杂交（smFISH）是一种新的基因表达分析方法，能报告转录本丰度和空间定位。 但到目前为止，smFISH还不能实现基因组范围的分析。 如今，瑞士苏黎世大学的研究人员报告了一种策略，让smFISH也插上高通量的翅膀。

2024年11月6日 · 单分子荧光原位杂交（smFISH）是分子生物学中用于检测RNA分子在细胞中定位和表达的技术。 它基于原位杂交技术，使用荧光标记的探针与目标RNA结合，形成荧光信号，通过显微镜观察可以观察到RNA的位置和数量。

Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to the target RNA ( Raj et al., 2008 ; Lee et al., 2016a ). We ...