2017年10月13日 · We have reported the application of selective optimized flip angle short transient (SOFAST) technique coupled to heteronuclear multiple quantum correlation (HMQC) along with nonlinear sampling (NUS) in urine and serum samples.

We have reported the application of selective optimized flip angle short transient (SOFAST) technique coupled to heteronuclear multiple quantum correlation (HMQC) along with nonlinear sampling (NUS) in urine and serum samples.

Guidelines are provided on how to set up SOFAST-HMQC experiments for a given protein sample. In addition, an alternative pulse scheme, IPAP-SOFAST-HMQC is presented that allows application on NMR spectrometers equipped with cryogenic probes, and fast measurement of one-bond 1H-13C and 1H-15N scalar and residual dipolar coupling constants.

2020年8月3日 · Here, we present the XL-ALSOFAST- [13 C, 1 H]-HMQC experiment with much improved sensitivity for fully protonated high molecular weight proteins. For the tested systems ranging from 100 to 240 kDa in size, 3-fold higher sensitivity was obtained on average for fast relaxing signals compared to current state-of-the-art experiments.

Go to an existing SOFAST experiment (pulse program: sofast), type “edc” to copy its parameters and generate a new experiment number. Or "rpar SFHMQCF3GPPH" in an new experiment entry. Type “ ased ” to adjust power length and power level for both 1H and 15N, including, pL1, p3, pL2, pL12, and pcpd2.

After the pulse z-magnetization is recovering with a typical rate, the T1-relaxation time. This magnetization is used for the next pulse and the relation between the pulse angle α and the ratio T/T1 is important α. A.Ross, M. Salzmann, H. Senn J. Biomol. NMR 10, 389-396 (1997) 1 has an influence on the repetition rate.

The main features of the SOFAST HMQC experiment are (1) the use of shaped 1 H pulses that manipulate only a subset of proton spins in order to enhance their spin–lattice relaxation (T 1) via dipolar interactions with the unperturbed proton spins; and (2) Ernst angle excitation to achieve maximal sensitivity for high repetition rates of the pulse...

We have reported the application of selective optimized flip angle short transient (SOFAST) technique coupled to heteronuclear multiple quantum correlation (HMQC) along with nonlinear sampling (NUS) in urine and serum samples.

We have re-ported the application of selective optimized flip angle short transient (SOFAST) technique coupled to heteronuclear mul-tiple quantum correlation (HMQC) along with nonlinear sam-pling (NUS) in urine and serum samples.

An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl 1 H− 13 C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds.