In human cells, the unique ogt gene in chromosome X encodes three isoforms of OGT (termed nucleocytoplasmic OGT (ncOGT), mitochondrial OGT (mOGT), and short-form (sOGT)) via post-transcriptional RNA processes. ncOGT and sOGT locate in the nuclear and cytoplasm in cell, whereas mOGT exists in mitochondria. Each isoform has an identical C ...

2022年4月25日 · O-linked N-acetylglucosamine transferase (OGT), a key protein in O-linked N-acetylglucosamine (O-GlcNAc) post-translational modification, catalyzes O-GlcNAcylation by linking GlcNAc from glycosyl donors to protein Ser/Thr residues to …

2012年4月23日 · O -GlcNAc transferase (OGT) catalyses the addition of O -linked β- D - N -acetylglucosamine (O -GlcNAc) to Ser or Thr residues on target substrates, whereas O -GlcNAcase (OGA) catalyses O...

蛋白质O-GlcNAc糖基化修饰，由O-GlcNAc转移酶(OGT)和O-GlcNAc水解酶(OGA)共同维持细胞内糖基化水平稳定，动态调节细胞信号转导途径中多种酶的功能，在许多生命活动中发挥着重要调节作用，并与多种代谢性疾病，如妊娠期糖尿病等的发生密切相关。

2017年5月10日 · Potential mechanisms that enable a single OGT enzyme to recognize hundreds of protein substrates include substrate-specific interactions with the tetratricopeptide repeat (TPR) domain of OGT...

In this review, the functions of GlcNAcylation in regulating cellular processes, its extensive crosstalk with protein phosphorylation, and regulation of OGT and O-GlcNAcase will be explored. GlcNAcylation rivals phosphorylation in terms of its abundance, protein distribution and its cycling on and off of proteins.

2003年1月15日 · O-Linked N -acetylglucosamine (GlcNAc) transferase (OGT) mediates a novel hexosamine-dependent signal transduction pathway. Yet, little is known about the regulation of ogt gene expression in mammals. We report the sequence and characterization of the mouse ogt locus and provide a comparison with the human and rat ogt genes.

体外研究表明,三种类型的OGT中sOGT的自身O-GlcNAc修饰现象最为显著,因此我们选择sOGT作为本文的研究对象.本文利用大肠杆菌表达系统及镍亲和层析方法得到了纯度较高,活性较好的sOGT蛋白;借助LC-MS,定点突变PCR,Western Blot等技术实现了sOGT蛋白上6个O-GlcNAc修饰位 …

2022年2月16日 · 本文利用大肠杆菌表达系统及镍 亲和层析方法得到了纯度较高、活性较好的sOGT蛋白；借助LCMS、定点突变 PCR、Western Blot等技术实现了sOGT蛋白上6个OGlcNAc修饰位点 (T12，S1 8 T38，$52，T449，T662)的定位；分别以十七肽CKII、Nup62蛋白为底物初步探索 了OGlcNA. 3、c修饰对sOGT功能的影响。

OGT是由X染色体上的一个基因通过选择性剪接形成三种亚型:100 kDa的ncOGT(nuclear cytoplasmic OGT);103 kDa的mOGT(mitochondrial OGT);78 kDa的sOGT(short OGT);它们有着相同的C端催化结构域;但是负责和靶蛋白结合和定位的N端结构域不相同。