2006年10月29日 · To monitor constitutive and regulated protein interactions within living mammalian cells, we have developed a biological assay termed split TEV. We engineered …

The split TEV technique [1] is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of two previously inactive fragments derived …

2024年1月1日 · In the split TEV interaction screen, we demonstrated that TAOK2 interacted with core kinase cassette components STK3, STK4, LATS1, LAT2, and MOB1A, implicating a key …

tev蛋白酶片段的功能重建可以使用“仅蛋白水解”的报道分子进行监测，该报道分子可以是以前沉默的荧光和发光报道蛋白。 此外，可以通过蛋白水解切割的非活性转录因子与所选的任何下游 …

2012年4月1日 · Recently, a protein complementation method named split-TEV (tobacco etch virus) has been developed and is based on the functional reconstitution of TEV protease and …

2021年6月5日 · Split-TEV assay enables the identification of protein–protein interaction in mammalian cells. This method is based on the split of tobacco etch virus (TEV) protease in …

To monitor constitutive and regulated protein interactions within living mammalian cells, we have developed a biological assay termed split TEV. We engineered inactive fragments of the NIa …

Split-protein methods have been effectively utilized for detecting protein-protein interactions in cell-free systems, E. coli, yeast, mammalian cells, plants, and live animals.

2023年10月7日 · Here, we employed a split TEV-based protein-protein interaction screen to assess the physical interactions among 28 key Hippo pathway components and potential …

tevprotease(tobaccoetchvirusprotease)是来源于烟草蚀纹病毒(tev)的一种半胱氨酸蛋白酶，基于tev蛋白酶的强底物特异性以及高活性，新近建立起一种可用于研究蛋白间相互作用的split-tev …