2024年1月23日 · Detection: incubate sections in streptavidin-HRP in PBS for 30 minutes at room temperature.

The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. It is a metalloenzyme with many isoforms, of which the most studied type is C. It catalyzes the oxidation of various organic substrates by hydrogen peroxide.

Horseradish peroxidase (HRP) is a 44-kDa protein that catalyzes the oxidation of substrates in the presence of hydrogen peroxide, resulting in a colored product or the release of light as one product of the reaction (chemiluminescence). HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides.

SignalStain ® Boost IHC Detection Reagent (HRP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

VECTASTAIN® Elite® ABC-HRP Kit, Peroxidase (Standard) (PK-6100) The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit. It is available without a biotinylated secondary antibody (Standard kit).

2019年4月1日 · A range of substrates is available for detection of cells labeled with horseradish peroxidase (HRP). Diaminobenzidine (DAB) is the most commonly used substrate and one of the most sensitive. It yields an intense brown product that is insoluble in both water and alcohol. DAB staining is compatible wi …

2019年7月8日 · 最為常用的酵素包括能將 DAB (3,3'-Diaminobenzidine) 呈色劑轉化為棕色產物的 HRP (Horseradish peroxidase) 酵素，以及能將 AEC (3-Amino-9-ethylcarbazole) 呈色劑轉化為紅色產物的 AP (Alkaline phosphatase) 酵素。

A range of substrates is available for detection of cells labeled with horseradish peroxidase (HRP). Diaminobenzidine (DAB) is the most commonly used substrate and one of the most sensitive. It yields an intense brown product that is insoluble in both water and alcohol.

4 天之前 · ReadiUse™ Stayright™ Purple Peroxidase (HRP) Substrate is suitable for use in peroxidase (HRP)-based immunohistochemistry (IHC) and in situ hybridization (ISH) staining methods. Upon HRP-induced oxidation, Stayright™ Purple forms a purple insoluble precipitating product at the target site of your assay.

-Histofine® High Stain TM HRP (MULTI) is a 2 Step Polymer Detection System to IHC staining providing more amplified staining intensity compared with conventional 1 step polymer detection system. This system is applicable to both of mouse and rabbit primary antibodies and is for formalin-fixed, paraffin-embedded tissue sections.