2020年6月1日 · Here, we show that direct contact between cancer cells and CAFs triggers the expression of a wide range of inflammatory modulators by fibroblasts. This is initiated following transcytosis of...

2023年2月1日 · In this study, we confirmed that GPR162, an orphan receptor in the G-protein-coupled receptor family, acted as a novel radiotherapy sensitizer by interacting with the stimulator of interferon genes...

We have used several different methods including luciferase assays, RT-qPCR, ELISA, electrophoretic detection of STING-α aggregates and biological assays for antiviral activity to demonstrate the dominant inhibitory effect of STING-β in innate nucleic acid sensing.

2024年1月13日 · cgas-sting信号通路是一种细胞内dna感应途径，在抗癌免疫应答期间激活i型干扰素（ ifn ）、促炎细胞因子和趋化因子的表达。因此，本研究探究了gpx4在coad中调节sting介导的干扰素信号传导的新机制。

2024年12月24日 · cGAS-like receptor (cGLR)-stimulator of interferon genes (STING) recently emerged as an important pathway controlling viral infections in invertebrates. However, its exact contribution at the organismal level remains uncharacterized. Here, we use STING::GFP knockin reporter Drosophila flies to document activation of the pathway in vivo.

1 天前 · Quantification of the mRNA levels of the ISGs Mx1, OAS1 and RSAD2 using qPCR indicated that STING was required for cGAMP signalling, both in ARF1 WT and R19C fibroblasts (Figs. 5F and EV5A). As expected, induction of ISGs by the RIG-I agonist polyI:C and the TLR4 agonist LPS was not dependent on STING (Fig. EV5B).

2020年6月15日 · Here, we show that cellular redox homeostasis maintained by glutathione peroxidase 4 (GPX4) is required for STING activation. GPX4 deficiency enhanced cellular lipid peroxidation and thus...

2024年7月14日 · SN-011对STING表达水平抑制效率验证. RT-qPCR检测显示，IRI组与Sham组相比，肾组织中STING的mRNA水平升高（P<0.05）；与IRI+DMSO组的差异无统计学意义（P>0.05）；与IRI组相比，SN-011可明显抑制STING mRNA水平（P<0.05，图4B）；Western blotting和免疫组织化学检测显示，与Sham组相比 ...

2024年9月20日 · With Cu-DPPZ-Py + and Cu-Elesclomol, there is strong evidence that the triggering cuproptosis significantly drives mitochondrial DNA (mtDNA) release to activate innate immunity via cyclic GMP-AMP synthase-stimulation of interferon genes (cGAS-STING), which can improve T cell antitumor immunity in vivo. By contrast, it is observed that Cu-DPPZ ...

2022年6月17日 · The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe a protocol to activate the cGAS-STING pathway in murine cells using nucleic acids transfection.