技术介绍 | Suntag系统在CRISPR系统中的应用 - 知乎
基于suntag系统的碱基编辑器 BE-Plus 的核心逻辑是在Cas9上融合多个GCN4，而将scFV融合于UGI和APOBEC。 多个UGI-APOBEC融合蛋白能够被GCN4富集，这样不仅可以提高碱基编 …
A protein-tagging system for signal amplification in gene …
2014年10月23日 · We also use the SunTag to create a potent synthetic transcription factor by recruiting multiple copies of a transcriptional activation domain to a nuclease-deficient …
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag ...
2019年2月13日 · Here, we adapt the dCas9-SunTag system to engineer targeted gene activation and DNA methylation in Arabidopsis. We demonstrate that a dCas9-SunTag system utilizing …
使用 dCas9-SunTag 系统和明亮的荧光蛋白对基因组位点进行活细 …
基于核酸酶失活的 Cas9，称为 dCas9，CRISPRCas9 系统可用作成像工具来标记基因组位点和可视化染色体的动态变化（Chen 等，2013；Cheng 等，2016；Fu 等， 2016；Ma 等 …
Targeted DNA demethylation in vivo using dCas9–peptide
2016年8月29日 · DNA methyl groups are selectively removed at target loci using inactive Cas9 fused to a SunTag-based peptide repeat that recruits the enzymatic activity.
Live cell imaging of genomic loci using dCas9-SunTag system and …
In this study, we combined CRISPR/Cas9 technology, SunTag system, and fluorescent proteins to image high-repetitive and low-repetitive chromosome loci in human cells. mNeonGreen …
技术介绍 | 基于Suntag的碱基编辑技术介绍 - 知乎
2024年2月23日 · 基于Suntag系统的碱基编辑器BE-Plus的核心逻辑是在Cas9上融合多个Suntag，而将scFV融合于 APOBEC。 这样，多个APOBEC融合蛋白便能够同时集中于靶位 …
DNA epigenome editing using CRISPR-Cas SunTag-directed …
2017年9月18日 · We demonstrate that dCas9-SunTag-DNMT3A dramatically increases CpG methylation at the HOXA5 locus in human embryonic kidney (HEK293T) cells. Furthermore, …
Comparison of Cas9 activators in multiple species - Nature
2016年5月23日 · Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the …
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag ...
2019年2月13日 · Here, we adapt the dCas9-SunTag system to engineer targeted gene activation and DNA methylation in Arabidopsis. We demonstrate that a dCas9-SunTag system utilizing …