DNA supercoiling refers to the amount of twist in a particular DNA strand, which determines the amount of strain on it. A given strand may be "positively supercoiled" or "negatively …

2024年8月1日 · Supercoiled DNA is the desired species when isolating plasmid DNA. DNA found in the supercoiled form is not easily accessed by the replication machinery. During replication, …

With the recent revival of the promise of plasmid DNA vectors in gene therapy, a novel synthetic biology approach was used to enhance the quantity, (yield), and quality of the plasmid DNA. …

In this paper we present a method (SCFluo) to monitor SC content in DNA solutions that is simple, rapid and requires small amounts of sample. SCFluo takes advantage of two well-documented …

2024年6月11日 · In this study, we observed a clear correlation between lower supercoiled DNA percentages in plasmid DNA and reduced mRNA purity. Heat-induced nicks in the supercoiled …

To obtain significant amounts of almost fully supercoiled DNA, we modified the standard protocol of a commercially available Qiagen plasmid purification kit. Our changes led to isolation of …

2021年9月28日 · DNA in cells is supercoiled and constrained into loops and this supercoiling and looping influence every aspect of DNA activity. We show here that negative supercoiling …

2017年7月12日 · Supercoiled pDNA is purified by multimodal chromatography with Capto™ adhere resin. 2-step elution with NaCl is used to remove open circular pDNA and RNA from sc …

Typically plasmid DNA molecules are used to measure DNA supercoiling status inside bacterial cells. In this chapter, we describe how to isolate plasmid DNA molecules from E. coli cells and …

2022年2月21日 · We use two model DNA plasmid systems, pFLIP-FUSE and pUC19, to study the dynamics of supercoiling-induced secondary structural transitions after perturbations away …