2022年11月6日 · Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer. Superfolder GFP was derived from Folding Reporter GFP with the following mutations: S30R/Y39N/N105T/Y145F/I171V/A206V.

提供 pCAMBIAsuper1300-GFP 载体/质粒图谱，全长序列，抗性，大小和元件信息。

2023年12月27日 · Superfolder GFP超折叠绿色荧光蛋白，简称sfGFP，是2005年发表的一种碱性（组成型荧光）绿色荧光蛋白。sfGFP来源于Aequorea victoria，分子量约为26.8kDa。sfGFP是一种非常快速成熟的弱二聚体。 绿色荧光蛋白GFP的现有变体在与其他蛋白融合表达时常常会错误折 …

2005年12月20日 · Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP,...

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble ...

Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides.

Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)

当与其他蛋白质融合表达时，绿色荧光蛋白（gfp）的现有变体通常会错折叠。 我们已经生成了一种牢固折叠的GFP版本，称为“超级文件夹” GFP，即使与折叠不良的多肽融合在一起也可以很好地折叠。

2005年9月20日 · Engineering and characterization of a superfolder green fluorescent protein. Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides.

Superfolder GFP (sGFP) is a variant of the Green Fluorescent Protein that folds efficiently when fused to poorly folded proteins. In this study, we show that sGFP, but not enhanced GFP, is functional in vivo at 70 degrees C in the extreme thermophile Thermus thermophilus (Tth); thus, permitting the …