D and E, chromatin immunoprecipitation of human (D) and mouse (E) SYN1 promoters was performed from SH-SY5Y and N2a cells, respectively. The PCR-amplified regions are boxed …

When probes were incubated with nuclear extracts from human SH-SY5Y or murine N2a cells, the formation of slower migrating DNA-protein complexes was observed (Fig. 1, B and C , lane 1 ). …

2020年8月31日 · SH-SY5Y cells, with inducible expression of human α-Syn (under the control of Tet-off response element), were used to investigate the potency of exogenously applied …

2022年6月28日 · Optogenetically modified SH-SY5Y cells were highly viable (>85%) after lentiviral transduction and neuronal differentiation. The cells demonstrated properties of …

Results: Sp1 binds to the SYN1 promoter, activating its transcription. Conclusion: Sp1 is a novel regulator of SYN1 transcription, whose activity is inhibited by REST and CpG methylation. …

2016年8月15日 · SH-SY5Y, a human neuroblastoma and a cellular model of neurodevelopment, consistently expresses genes related to neurodegenerative disorders at different biological …

Human and mouse SYN1 promoter sequences were cloned in the pGL3 reporter vector and transiently co-transfected in SH-SY5Y and N2a cells, respectively. When Sp1 expression was …

2023年6月14日 · We observed that complementation in human embryonic kidney (HEK) 293 or SH-SY5Y cells led to the formation of fluorescent puncta (Fig. 1A and fig. S1B), suggesting …

The phosphoprotein synapsin I (Syn I) plays a crucial role during axonogenesis and synaptogenesis as well as in synaptic transmission and plasticity of mature neurons. …

2013年2月1日 · D and E, chromatin immunoprecipitation of human (D) and mouse (E) SYN1 promoters was performed from SH-SY5Y and N2a cells, respectively. The PCR-amplified …