2021年4月19日 · SH-SY5Y cells can be differentiated into neuron-like cells (SH-SY5Y-derived neurons) using various substances. This review evaluates whether SH-SY5Y-derived neurons are a suitable model for (i) investigating intracellular TAU sorting in general, and (ii) with respect to neuron subtype-specific TAU vulnerability.

2024年3月15日 · 采用免疫印迹、免疫荧光和免疫组化染色分别验证C004019在细胞模型 (HEK293和SH-SY5Y)和小鼠模型 (hTau -转基因和3xTg-AD)中的作用。 通过一系列的行为实验来评估小鼠的认知能力。 电生理和高尔基染色评价突触可塑性。 结果： 在稳定或短暂过表达人tau (hTau)的HEK293细胞中，C004019通过促进其泛素化-蛋白酶依赖性的蛋白水解，诱导了tau蛋白的强劲清除SH-SY5Y细胞中组成性过表达hTau。 此外，C004019脑室灌注诱导了体内tau蛋白 …

2019年5月1日 · New method: In order to model phosphatase downregulation and analyze its effect on tau aggregation in vitro, we treated neuroblastoma SH-SY5Y cells with okadaic acid (OA), a protein phosphatase inhibitor, and examined high molecular weight phospho-tau species.

1997年10月17日 · Here we report three experimental paradigms in which tau proteins are differentially localized and expressed in human neuroblastoma cells SH-SY5Y. We found that in undifferentiated cells, tau proteins were predominantly localized in the nucleus.

探讨外源性三价铁离子对人神经母细胞瘤细胞（SH-SY5Y细胞）活性及tau过度磷酸化和聚集的影响。 采用Fe 3+ 浓度为10、100、200和400 mg·L −1 的三氯化铁（FeCl 3）处理SH-SY5Y细胞，通过CCK8法检测细胞存活率。 用10和200 mg·L −1 染毒24 h，通过普鲁士蓝（Perl's）铁染色法测定胞内铁含量。 转染tau-P301L质粒构建tau过表达的AD样细胞模型，10、200 mg·L −1 染毒SH-SY5Y细胞及tau过表达的SH-SY5Y细胞24 h后，采用免疫印迹检测磷酸化tau（p-tau）蛋白表 …

2024年3月22日 · Here, we describe and discuss a general cultivation procedure as well as four differentiation methods to obtain SH-SY5Y-derived neurons resembling noradrenergic, dopaminergic, and cholinergic properties, based on the treatment with retinoic acid (RA), brain-derived neurotrophic factor (BDNF), and 12-O-tetrade canoylphorbol-13-acetate (TPA).

氯化铁(FeCl3) 处理SH-SY5Y 细胞,通过CCK8 法检测细胞存活率�. 24 h 后, 采用免疫印迹检测磷酸化tau(p-tau)蛋白表达的差异。将磷酸缓冲盐溶液(PBS) 稀释后的FeCl3 、人源性tauR3 及FeCl3 和tauR3共同在37 °C孵育, 通过�. 不需要利益冲突 无申报收稿日期. Y 细胞24 h. 黄褐色, 且200 mg·L−1 染毒组阳性细胞率增�. 0.01)。FeCl3 染毒细胞后SH-SY5Y. 性三价铁离子对SH-SY5Y 细胞tau�. 白磷酸化和聚集的影响[J]. 环境与. ,200 mg·L−1 染毒组p-tau(Ser396)蛋白表达量上 …

2021年6月14日 · Here we report a genome-wide CRISPR screen to identify modulators of endogenous tau protein for the first time. Primary screens performed in SH-SY5Y cells, identified positive and negative...

2025年3月13日 · Proteasomal inhibition induces an increase in pTau (Thr231)/TAU in SH-SY5Y cells. pTau (Thr231) and Tau protein levels were evaluated in SH-SY5Y cells by western blot. Cells were treated with aggregated Aβ1–42, RT, Olig and MG-132. pTau (Thr231) and Tau were detected by subjecting the cellular lysates to western blot analysis with specific ...

We found that the OA induced tau phosphorylation at proline-directed site (Thr 205) in SH-SY5Y cells can be prevented by 17β-estradiol in a dose-dependent manner. A 50% reduction in the effects of OA were observed by the 100 nM concentration of E2, and 10 M E2 completely prevented tau hyperphosphorylation induced by OA.