描述：T7 gp4 蛋白来源于T7 噬菌体，是其复制系统的一 种核心蛋白，它既具有引发酶的作用也具有解旋酶的活 性，主要应用于全基因组扩增（WGA），且在扩增环状

2022年8月1日 · The replicative system from bacteriophage T7 provides a model system for studying DNA replication. T7 gp4 encodes a dual functional protein with primase on its N-terminal domain (NTD) and SF4 helicase on its C-terminal domain.

2020年4月6日 · We propose that a hypothetical intermediate conformation of the gp4-DNA complex during DNA unwinding can help explain how gp4 molecules pass lesions, providing insights into the unwinding dynamics of gp4. 六边形T7解旋酶（gp4）采用螺旋锁紧垫圈形式，并围绕一个盘绕状DNA（跟踪）链，每个亚基结合了两个核苷酸。 但是，放卷中的化学机械耦合机理尚未阐明。 在这里，我们利用具有一个核苷酸精度的纳米张紧器增强的Förster共振能量转移 …

2012年10月5日 · Bacteriophage T7 expresses two forms of gene 4 protein (gp4). The 63-kDa full-length gp4 contains both the helicase and primase domains. T7 phage also express a 56-kDa truncated gp4 lacking the zinc binding domain of the primase; the protein has helicase activity but no DNA-dependent primase activit …

2013年3月4日 · We show here that the Arabidopsis T7 gp4 homologue has both DNA primase and DNA helicase activities, the first such report from a higher eukaryote. The gene for this protein is highly expressed in rapidly growing plant tissues and is correlated with organelle DNA polymerase gene expression.

2020年2月12日 · T7gp4蛋白来源于T7噬菌体，是其复制系统的一种核心蛋白，它既具有引发酶的作用也具有解旋酶的活性，主要应用于全基因组扩增（WGA），且在扩增环状全基因组上有非常大的优势，常用于病毒检测。

T7 gp4蛋白来源于T7噬菌体，是其复制系统的一种核心蛋白，它既具有引发酶的作用也具有解旋酶的活性，主要应用于全基因组扩增（WGA），且在扩增环状全基因组上有非常大的优势，常用于病毒检测。

2019年6月6日 · We examined the interactions between three of these proteins from Arabidopsis thaliana: a DNA helicase-primase similar to bacteriophage T7 gp4 protein and animal mitochondrial Twinkle, and two DNA polymerases, Pol1A and Pol1B. We used a three-pronged approach to analyze the interactions, including Yeast-two-hybrid analysis, Direct Coupling ...

2010年5月18日 · Gene 4 protein (gp4) encoded by bacteriophage T7 contains a C-terminal helicase and an N-terminal primase domain. After synthesis of tetraribonucleotides, gp4 must transfer them to the polymerase for use as primers to initiate DNA synthesis.

2022年8月8日 · The replicative system from bacteriophage T7 provides a model system for studying DNA replication. T7 gp4 encodes a dual functional protein with primase on its N-terminal domain (NTD) and SF4 helicase on its C-terminal domain.