Plasmid T7opt in pCAGGS from Dr. Benhur Lee's lab contains the insert T7-opt and is published in J Virol. 2015 Jan 15;89(2):1242-53. doi: 10.1128/JVI.02583-14. Epub 2014 Nov 12. This plasmid is available through Addgene.

T7 Plasmid Expression System Features. Bacterial T7 promoter-based vectors allow for expression, detection and purification of recombinant FLAG ® and MAT™ (Metal Affinity Tag) fusions in E. coli. Several vectors containing the T7 promoter offer dual tag options for FLAG ® and MAT™-tagged fusion proteins. These vectors confer ampicillin ...

Plasmid T7-VEE-GFP from Dr. Steven Dowdy's lab contains the insert EGFP and is published in Cell Stem Cell. 2013 Aug 1;13 (2):246-54. doi: 10.1016/j.stem.2013.06.001. This plasmid is available through Addgene.

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T7 RNA polymerase is responsible for beginning transcription at the T7 promoter of the transformed vector. The T7 gene is itself under the control of a lac promoter. Normally, both the lac promoter and the T7 promoter are repressed in the E. coli cell by the Lac repressor.

Yeast two-hybrid "bait" vector for expressing proteins fused to the GAL4 DNA-binding domain. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets. Sticky ends from different DraIII sites may not be compatible. Efficient cleavage requires at least two copies of the PaqCI recognition sequence.

T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.

A T7 expression plasmid containing a gene encoding E. coli UDG was transformed into each host, grown to 0.6 OD and induced for 3 hours. Comparison of soluble extracts from uninduced (-) and induced (+) cells shows superior control of expression in the T7 Express hosts while maintaining high levels of induced expression.

2024年10月1日 · Overall, we utilized a potential heat inducible promoter (HI) and combined it with the genetic amplifier, the plasmid-driven T7 (PDT7) system, to construct a stable, high-yield, and efficient platform for recombinant CA production.

2021年8月20日 · We present a novel type of plasmid, termed T7 ori plasmids that replicate, in an engineered Escherichia coli, with a T7 phage origin as the sole origin of replication. We find that while the T7 replication proteins; T7 DNA polymerase, T7 single-stranded binding proteins and T7 helicase-primase are dispensable for replication, T7 RNAP is ...