T7 RNA Polymerase - NEB
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a …
T7 RNA polymerase - Wikipedia
In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and …
T7 RNA聚合酶的前世今生 - 知乎 - 知乎专栏
噬菌体T7 RNA聚合酶 （T7 RNA Polymerase，T7 RNAP）于1970年首次从噬菌体T7感染的大肠杆菌细胞中分离出来，是催化RNA合成的最简单的酶之一。 T7噬菌体 是一种中等大小的噬菌 …
T7 RNA聚合酶-T7 RNA Polymerase - Takara
本酶是噬菌体T7 DNA编码的酶，以含有T7启动子序列的双链DNA为模板，以NTP为底物，合成与启动子下游的单链DNA互补的RNA。 本酶对T7启动子序列具有高度特异性，不能识别其它生 …
T7 RNA聚合酶-T7启动子是什么？ - 知乎专栏
2024年3月20日 · T7 RNA聚合酶，(T7 RNA Polymerase) 是一种高度特异识别T7启动子序列的DNA依赖的5'→3' RNA聚合酶。 T7 RNA Polymerase以含有T7启动子序列的单链或双链DNA …
T7 RNA Polymerase - an overview | ScienceDirect Topics
Despite its structural simplicity compared with the multimeric prokaryotic and eukaryotic RNA polymerases, T7 RNA polymerase (Pol) performs a highly specific promoter recognition and all …
The highly efficient T7 RNA polymerase: A wonder …
2018年10月15日 · The lysozyme of bacteriophage T7 is a multifunctional zinc amidase protein that can perform cell lysis during phage infection by cutting amide bonds in the bacterial cell …
Engineered T7 RNA polymerase reduces dsRNA formation by …
2025年3月3日 · By combining the molecular beacon-based fluorescence-activated droplet sorting (FADS) utilized for random library screening with site-directed mutagenesis aimed at …
T7 RNA Polymerase: Mechanism and Engineered Variants
2025年2月11日 · T7 RNA polymerase is a monomeric enzyme with a compact structure crucial for rapidly transcribing phage DNA. Comprising a single polypeptide chain of approximately 883 …
BPS2025 - T7 RNA polymerase—fundamental principles drive utility
2025年2月13日 · The single subunit T7 RNA polymerase is both a model for mechanisms in transcription, in general, and a work horse for RNA synthesis and manufacturing. A deep …