2015年5月26日 · The T7 tag is the first 11 amino acids of the T7 gene 10 protein. Basically, if you engineer a protein with the T7 tag, you've engineered a small, known epitope to the protein of …

2015年5月26日 · The T7 tag already starts with methionine and naturally functions to initiate translation of T7 gene10. pET based vectors also contain it for this purpose. In this vector, …

t7-promoter currently has no approved synonyms. see all tag synonyms » Users with more than 2500 reputation and a total answer score of 5 or more on the tag, can suggest tag synonyms. …

2014年7月5日 · What is the pBR322 convention? For example in the description of the pET28a vector we can find information that the sequence is numbered by the pBR322 convention, so …

Q&A for biology researchers, academics, and students. Stack Exchange Network. Stack Exchange network consists of 183 Q&A communities including Stack Overflow, the largest, …

Q&A for biology researchers, academics, and students. Stack Exchange network consists of 182 Q&A communities including Stack Overflow, the largest, most trusted online community for …

2015年10月17日 · To design a experiment in feeding of C. elegans. It has to choose a plasmid vector to insert the gene of interest that can feed to C. elegans. Many paper are using pL4440 …

2014年6月10日 · I'm trying to purify the protein by Ni-NTA affinity chromatography. It seems that my protein (size about 54 kDa) is co-purified with chaperone protein (probably GroEL - as the …

2012年6月27日 · Can a gene be expressed under the T7 promoter in an E. coli strain (e.g. DH5 alpha), which does not have the T7 polymerase gene encoded in its genome? In other words, …

It's maybe a stupid question, but I really want to know the answer. The definition of Promoter (From Wikipedia): In genetics, a promoter is a region of DNA that initiates transcription of a