An expression vector, most commonly the pET expression vector, is engineered to integrate two essential components: a T7 promoter and a gene of interest downstream of the promoter and under its control. The expression vector is transformed into one of several relevant strains of E. coli, most frequently BL21 (DE3).

Within the realm of E. coli expression, the T7 system is the most popular approach for producing recombinant protein. In this system, the target gene is cloned into an expression vector downstream of the T7 promoter and this construct is introduced into a T7 expression host.

Bacterial T7 promoter-based vectors allow for expression, detection and purification of recombinant FLAG ® and MAT™ (Metal Affinity Tag) fusions in E. coli. Several vectors containing the T7 promoter offer dual tag options for FLAG ® and MAT™-tagged fusion proteins.

Promoter for bacteriophage T7 RNA polymerase. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different SfcI sites may not be compatible. SfcI …

pSNAP-tag (T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the …

The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21 (DE3) E. coli. These cells also produce T7 lysozyme to r

Vector with a traditional MCS, for bacterial or cell-free expression of a protein with a cleavable N-terminal 6xHis-HaloTag® dual tag.

Flexi® vector with an ampicillin resistance marker, for bacterial or in vitro expression of an untagged protein.

pH6HTC His6HaloTag ® T7 Vector (Cat.# G8031) 用于大肠杆菌和T7无细胞表达系统中具有C- 末端His6-HaloTag ® 双标签蛋白的表达。 注意： Flexi ® 载体带有致死性 barnase 基因，可以减少载体构建过程 中不带有插入片段的阴性克隆的产生。 barnase 基因可被插入的目的 DNA 片段所替代。 在没有插入目的DNA 片段的情况下，购买的此类载体无法在常用的实验用大肠杆菌菌株中生长。 Promega公司是为生命科学产业提供创新型解决方案和技术支持的领导者。 公司拥 …

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the ...