Slow TCA flux and ATP production in primary solid tumours but …
2023年2月1日 · Here we developed and validated a strategy to measure TCA cycle flux in tissues and tumours in mice, using isotope tracer infusion, mass spectrometry (MS) and quantitative …
Nature重磅发现：肿瘤的代谢比预想得更慢，为生长和转移保存能 …
2023年2月13日 · 在这项最新研究中，研究团队开发并验证了一种测量小鼠正常组织和肿瘤组织中线粒体三羧酸循环 （tca循环） 通量的策略，即注射同位素示踪剂，再通过质谱和由此产生的 …
Glucose feeds the TCA cycle via circulating lactate - Nature
2017年10月18日 · To quantitatively delineate the direct flux contributions to the TCA cycle by circulating glucose, lactate, and glutamine, we developed a linear algebra model to compute …
Regulation and function of the mammalian tricarboxylic acid cycle
Metabolic flux through the TCA cycle is tightly coordinated by both negative and positive allosteric regulation of TCA cycle–associated enzymes. Three enzymes in particular—CS, IDH, and …
Nature | 实体瘤的产能速率更低？！ - 知乎专栏
2023年2月1日，来自美国普林斯顿大学的Joshua D. Rabinowitz研究团队在Nature上发表题为Slow TCA flux and ATP production in primary solid tumours but not metastases的文章，发现在所检 …
Lactate transported by MCT1 plays an active role in promoting ...
2024年6月26日 · Our metabolic flux analysis reveals that skeletal muscle mainly uses lactate to fuel TCA cycle. Lactate is transported through the cell membrane via monocarboxylate …
Quantitative Fluxomics of Circulating Metabolites - Cell Press
2020年10月6日 · By infusing 15 different 13C-isotope tracers, Hui et al. quantify metabolic fluxes in mice on either high-carbohydrate or ketogenic diet. Results include interconversion fluxes …
Assessing cancer therapeutic efficacy in vivo using [2H7]glucose
2 天之前 · Analysis revealed treatment-induced decreases in glycolytic flux without substantially altering tricarboxylic acid (TCA) cycle turnover . In addition, global metabolomics analysis with …
Quantitative Analysis of the Physiological Contributions of Glucose …
2020年9月21日 · Using isotope-labeling data in vivo across several experimental conditions, we construct multiple models of central carbon metabolism and develop methods based on …
Hierarchical tricarboxylic acid cycle regulation by hepatocyte …
Stable isotope labeling data now demonstrate a hierarchical relationship whereby urea cycle flux limits TCA cycle function and, ultimately, downstream mitochondrial oxidative metabolism. The …