2017年6月19日 · We examined the spatial expression patterns of these TCP genes using histochemical GUS staining analysis. GUS activity in TCP3:GUS, TCP4:GUS, and TCP10:GUS plants were detected in leaf vascular tissues where CO is expressed (Fig 3A to 3C, [9, 40, 57]), indicating that these TCP proteins could exist where CO transcription occurs.

2022年7月11日 · The TCP4pro-TCP4-GUS construct, in which a 2818-bp-long TCP4 promoter was used to drive a β-glucuronidase (GUS) reporter gene fusion with TCP4 genomic DNA, was transformed into Arabidopsis (Lan et al., 2021). Subsequently, stable TCP4pro-TCP4-GUS transgenic plants were used for GUS staining analysis.

2023年9月13日 · Here, we show that Class II TEOSINTE BRANCHED 1/CYCLOIDEA/ PCF (TCP) transcription factors redundantly protect ovule identity under HT. The duodecuple tcp2/3/4/5/10/13/17/24/1/12/18/16 (tcpDUO)...

2021年2月12日 · Our findings reveal a molecular framework in which TCPs suppress trichome formation on adaxial sides of cotyledons by repressing the activity of the MBW complex at the protein level and the transcripts of R3 MYB genes at the transcriptional level.

2019年5月31日 · To investigate the expression pattern of TCP5, we generated a TCP5pro-GUS construct in which the GUS reporter gene was driven by the 2,400-bp TCP5 promoter. Fifteen TCP5pro-GUS transgenic lines showed similar GUS staining patterns, and strong GUS activity was observed in the cotyledons, hypocotyls, young leaf blades, and petioles ( Figures 1 G ...

2023年12月8日 · TCP guns or Tubing Conveyed Perforating means transporting or conveying a perforating gun into a well via tubing, drill pipe, or coiled tubing. There are also TCP gun systems that are conveyed into the well via slickline or wireline.

2024年6月14日 · 2024年3月，华中农业大学园艺学院于Plant physiology期刊发表了题为“TCP transcription factor represses potato tuberization by regulating tuberigen complex activity”的研究论文。本研究报道了TCP家族转录因子分别与FT-like蛋白和蛋白互作从而调控马铃薯熟性的分子机 …

2011年7月14日 · AtTCP15 protein binds directly to the promoter regions of CYCA2;3 and RETINOBLASTOMA-RELATED (RBR) genes, which play key roles in endoreduplication. Taken together, AtTCP15 plays an important role in regulating endoreduplication during Arabidopsis development. Plant growth depends on coordinative cell proliferation and cell differentiation.

Using histochemical staining, GUS activity assay and quantitative RT–PCR analysis, we observed a dramatic enhancement in GUS expression in the 128 bp–GUS transformants, but, unexpectedly, this occurred in the absence of pathogen challenge. This was in contrast to the results observed with the 316 bp–GUS transformants,

2013年2月26日 · We identified the TCP Interactor containing EAR motif protein1 (TIE1), a novel transcriptional repressor, as a major modulator of TCP activities during leaf development. Overexpression of TIE1 leads to hyponastic and serrated leaves, whereas disruption of TIE1 causes epinastic leaves.