Structural insights into Ubr1-mediated N-degron polyubiquitination
2021年11月17日 · Here we developed chemical strategies to mimic the reaction intermediates of the first and second ubiquitin transfer steps, and determined the cryo-electron microscopy …
泛素连接酶E3 成分N- 识别蛋白1 （ubiquitin protein ligase E3 component N-recognin 1，Ubr1）已被证明是N端规则信号通路最重要的E3 连接酶，能识别含不稳定氨基酸末端的蛋 …
The N-degron protein degradation strategy for investigating the ...
1998年9月11日 · The rfa1td plasmid was linearized with Bcl I and transformed into the yeast strains SX46A and SX46A ubr1:: his3. Recombinants of ura3 + were selected and integration …
The N-end rule pathway is mediated by a complex of the RING-type Ubr1 …
2010年11月14日 · Substrates of the N-end rule pathway are recognized by the Ubr1 E3 ubiquitin ligase through their destabilizing amino-terminal residues. Our previous work showed that the …
Reprograming of the ubiquitin ligase Ubr1 by intrinsically …
2025年2月7日 · In this study, we take an in vitro reconstitution approach to understand Ubr1 reprograming by Roq1. We show that Roq1 differentially modulates the ubiquitination of …
Efficient protein depletion by genetically controlled deprotection …
2009年4月28日 · Degradation is dependent on the E3 protein, which is encoded by the ubiquitin‐protein ligase gene UBR1 (Figure 2A), indicating proteasomal degradation by the …
啤酒酵母Ubr1野生型和缺失型菌株的比较蛋白组学分析 - 百度学术
目的 寻找并验证Ubr1泛素化底物蛋白大规模筛选方法的可行性.方法 通过对啤酒酵母RJD347 (Ubr1野生型)和AVY26 (Ubr1缺失型)的差异蛋白质组学比较,分析酵母中可能存在的Ubr1直接 …
Substrate-binding Sites of UBR1, the Ubiquitin Ligase of the N …
We show here that the type-1/2 sites of UBR1, the sole N-recognin of the yeast Saccharomyces cerevisiae, are located in the first ∼700 residues of the 1,950-residue UBR1. These sites are …
The UBR-1 ubiquitin ligase regulates glutamate metabolism to …
2018年4月12日 · Here we found that the C. elegans UBR-1 regulates glutamate level. When UBR-1 is defective, C. elegans exhibits increased glutamate; this leads to synchronization of …
Ubr1 和 Ubr2 在降解未折叠胞质蛋白的质量控制途径中起作用 …
2010年5月12日 · 我们的研究结果基于该质量控制系统的效率，表明细胞会利用生长潜力来避免与未折叠或错误折叠蛋白质积累相关的潜在毒性。 因此，Ubr1 和 Ubr2 代表了在细胞溶质核糖 …
Proteins targeted for degra-dation are bound on their N-terminal residue by Ubr1 (also designated E3. a and N-recognin), which catalyzes the covalent attachment of ubiquitin to the protein …
UBR1 antibody (26069-1-AP) | Proteintech - ptglab.com
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 40x lens). The Proteintech guarantee covers …
营养感应调控脂肪变性的新机制 - 生物通
2023年6月2日，北京大学生命科学学院朱健/刘敏课题组在 Journal of Cachexia, Sarcopenia and Muscle 杂志发表了题为 “Identification of Ubr1 as an amino acid sensor of steatosis in liver and …
(PDF) Loss of protein quality control gene UBR1 sensitizes ...
2020年10月23日 · Ubr1 is a conserved ubiquitin ligase involved in the degradation of aberrant proteins in eukaryotic cells. The human enzyme is found mutated in patients with Johanson …
UBR-1 deficiency leads to ivermectin resistance in C. elegans
2025年2月21日 · We found that the functional loss of an evolutionarily conserved E3 ubiquitin ligase, UBR-1, leads to IVM resistance in Caenorhabditis elegans. Multiple IVM-inhibiting …
潘漫课题组在《Nature》报道解析泛素E3连接酶动态酶学机制的“ …
该文通过蛋白化学合成技术和冷冻电镜技术，开发了针对泛素E3酶的“化学捕获”策略，并揭示了泛素E3连接酶Ubr1识别并以K48链型多聚泛素化修饰底物的动态机制。
EMBO Reports | 高尚邦教授团队发现泛素连接酶UBR-1调控神经系 …
2023年10月15日 · 神经系统中兴奋/抑制（E/I）平衡对于调节大脑皮层信息流至关重要。 为了防止神经元过度活跃或静息，维持神经回路兴奋性和抑制性之间的动态平衡必不可少。 E/I失衡会 …
(PDF) Ubiquitin Ligases of the N-End Rule Pathway: Assessment of ...
2011年9月13日 · Ubr1 is a conserved ubiquitin ligase involved in the degradation of aberrant proteins in eukaryotic cells. The human enzyme is found mutated in patients with Johanson …
2023年10月9日 · An alternative and complementary approach to address JBS is through the characterization of genetic modifiers of phenotypes exhibited by an UBR1 animal model. This …
UBR1 Promotes Sex-Dependent ACE2 Ubiquitination in …
2024年11月27日 · Proteomics analysis of the hypothalamus identified UBR1 as a potential E3 (ubiquitin protein ligase) ligase promoting ACE2 ubiquitination. Enhanced UBR1 expression, …
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