Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia /lymphoma cells.

2025年3月1日 · Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable DNA synthesis activity, TdTs have promoted the development of numerous nucleic acid-based methods, tools, and associated applications ...

2017年9月13日 · Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase, meaning that it can add DNA bases to an existing single strand of DNA without the sequence being dictated by a longer complementary strand. TdT must have a starting strand of DNA 3 base pairs long, and it adds to the 3' end of the strand like most polymerases.

We compare and contrast the molecular events for template-independent DNA synthesis catalyzed by terminal deoxynucleotidyl transferase with other well-characterized DNA polymerases that perform template-dependent synthesis.

2018年12月1日 · Terminal deoxynucleotidyltransferase (TdT) is a member of the polX family which is involved in DNA repair. It has been known for years as an untemplated DNA polymerase used during V (D)J recombination to generate diversity at the CDR3 region of immunoglobulins and T-cell receptors.

2022年11月7日 · Terminal deoxynucleotidyl transferase (TdT) is a unique polymerase enzyme that does not require a DNA template in order to amplify DNA. The enzyme allows for the stepwise addition of single nucleotides onto the 3’ end of any oligonucleotide sequence. This has made TdT a very exciting molecular tool in biotechnology applications.

2002年2月1日 · The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 Å resolution reveals a typical DNA polymerase β-like fold locked in a closed form.

Thermal death time (TDT) cans 2.5 in. in diameter and 0.375 in. deep, designated as 208 × 006 in canning industry nomenclature, are used as con- tainers for food products for evaluating the effect of heat on microorganisms

2006年8月25日 · 'Correction for "thermal lag" (time lag required for internal food temperature to reach bath temperature when TDT tubes are plunged into the bath) and "lethality during lag" (thermal destruction...

This plot is called the thermal death time (TDT) curve ( figure 3). From such a plot, the thermal sensitivity of a microorganism, z, can be determined as the temperature change...