2023年12月1日 · Here, a Two-step tangential-flow filtration (TFF) method was successfully employed to improve the properties of mRNA-LNPs during the preparation process. This method involves an additional ethanol removal step prior to the particle fusion process.

2018年11月1日 · Here we report a single-step flash nanocomplexation (FNC) process using turbulent mixing to prepare uniform lipo-complex or lipo-polyplex LNPs in a scalable manner, demonstrating excellent control over the nanoparticle size (from 40 to several hundred nm) and surface charge, with narrow size distribution.

2 天之前 · stability conditions for a pDNA-LNP formulation Post-TFF. As demonstrated in the example above (Table 1), storage conditions have a significant impact on nucleic acid stability, leading to rapid degradation that ultimately reduces formulation potency. To mitigate this risk, the scientific team employs rigorous quality control measures and ...

该研究发现：在制备过程中，采用两步切向流过滤(tangential-flow filtration, TFF)方法可有效改善mRNA-LNPs的诸多理化性质，特别是在空包率、储存稳定性、转染效率以及体内分布方面。

2022年6月7日 · We have developed a unique platform to obtain any specific size-range of LNPs from 60 to 180 nm satisfying pharmaceutical regulatory requirements for polydispersity index, sterility, dose...

2022年5月19日 · 已经在国内多家测试成功，采用多次稀释-sptff浓缩即可以成功实现lnp的换液，对mrna-lnp结构实现了最大程度的保护。 图 颇尔SPTFF单向流动模式 mRNA-LNP的除菌过滤

LNP--浓缩&除醇&换液--TFF. 对于LNP体系中乙醇的去除通常需要以切向流过滤（Tangential-Flow Filtration,TFF）的方式，通过浓缩换液来实现（如图3）。而对于该环节滤器的应用考量，我们需要关注膜类型、材质、精度以及过滤工艺参数对其LNP自身特性指标的影响。

Our data demonstrate the efficacy of the Delta 100 kDa TFF membrane cassette for use in mRNA, saRNA, and LNP processing. In all processes, we observed no significant difference in NWP after DF or UF/DF processing indicating low membrane fouling.

脂质纳米颗粒 （LNP）最常用于mRNA传递，将低pH缓冲液渗滤到 中性缓冲液 中，并使用超滤浓缩颗粒。TFF步骤必须迅速，因为脂质可以在低pH下水解，导致形成杂质，例如作为可影响脂质双层结构，制剂稳定性和药物释放特性的水脂。

Repligen offers solutions that help overcome key challenges in mRNA/LNP, and pDNA manufacturing, with hands-on process and implementation consultation from recognized Gene Therapy, oncolytic and vaccines industry. Click on the workflow links to find productivity and throughput solutions you can implement today.