005698 B6.Cg-Tg(Gfap-TK)7.1Mvs/J Transgene-derived HSV-TK in these mice is present exclusively in cells expressing endogenous Gfap . This co-expression occurs in brain astrocytes and adult neural stem cells, enteric glia, hepatic stellate cells, and unknown cells in heart, lung, kidney, adrenal, and spleen.

2022年7月19日 · 转基因载体的标准表达式: Tg(promotor:gene) Tg表示普通转基因，括号内则记录了该载体的最关键特征成分，包括调控元件(例如: 启动子)以及有编码功能的基因，两者用冒号隔开 连续的调控元件或者融合读码框之间用横线连接

2006年10月2日 · To create a similar model for studies of glial cells in zebrafish, we have generated transgenic lines of zebrafish, Tg(gfap:GFP), that express GFP driven by glial specific regulatory elements from the zebrafish gfap gene. Here we describe the expression pattern of the GFP reporter in developing and adult Tg(gfap:GFP) mi2001 zebrafish.

012886 B6.Cg-Tg(Gfap-cre)73.12Mvs/J This strain is undergoing recovery from cryopreserved stocks. GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter.

Hemizygotes express Cre recombinase under the control of the human glial fibrillary acidic protein promoter ( GFAP ). When crossed with a strain containing <em>loxP</em> site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target, and recombination occurs primarily in the central nervous ...

我们已经生成了转基因斑马鱼，该斑马鱼在由斑马鱼神经胶质原纤维酸性蛋白（GFAP）调控元件驱动的神经胶质细胞中表达绿色荧光蛋白（GFP）。 Tg（gfap：GFP）转基因株系由三个创始人产生；此处显示的结果来自mi2001系列。 GFP的表达首先在尾芽期的活胚中可见，然后在发育中的大脑通过5次方铁素体阶段（受精后约12小时，hpf）可见，然后在发育中的脊髓中沿12子向后扩散-somite阶段（约15 hpf）。 在24hpf表达GFP的细胞在视网膜和晶状体中。 到72 hpf …

2022年4月30日 · In present study, we generated the Tg(gfap:EGFP) reporter line using medaka gfap regulatory elements containing approximately 2 kb 5′ upstream sequence from the translational initiation site and intron1 of medaka gfap. We identified the founder, which expressed EGFP in the eye, brain, and spinal cord in larval medaka, and confirmed EGFP co ...

We have generated transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. Transgenic lines Tg (gfap:GFP) were generated from three founders; the …

The Gfap promoter was used to drive cerebral expression of Il6. Several lines were created including lines G369, G16, and G167. G16 founder contains both the transgene and another transgene expressing lacZ under the control of Gfap whereas G26 and G36 founders do not.

该转基因小鼠品系在人胶质纤维酸性蛋白启动子 (GFAP) 控制下表达 Cre 重组酶。 当与含 loxP 位点侧翼序列的菌株交配时， cre 介导的重组导致目标的组织特异性缺失。