MLL-AF9 and MLL-AF4 oncofusion proteins bind a distinct
2017年1月23日 · In order to define a core set of MLL rearranged targets, we investigated the genome-wide binding of the MLL-AF9 and MLL-AF4 fusion proteins and associated epigenetic signatures in acute myeloid...
急性单核白血病细胞系THP-1细胞MLL-AF9融合基因沉默对p27表 …
目的 研究急性单核细胞白血病细胞系THP-1细胞MLL-AF9融合基因沉默对p27表达及转录调控的影响.方法 选择THP-1细胞特有的MLL-AF9融合基因为靶基因,设计并俣成MLL-AF9小干扰RNA(siRNA)片段.应用脂质体转染.方法 将MLL-AF9 siRNA导入细胞,通过流式细胞术检测siRNA转 …
Downregulation of DNA methylation enhances differentiation of THP-1 …
2023年8月12日 · MLL-AF9-driven AML-type leukemic THP-1 cells, which derived from acute monocytic leukemia (AMoL) patient, differentiate into macrophages upon treatment with phorbol-12-myristate-13-acetate...
Cytogenetic and molecular analysis of the acute monocytic ... - PubMed
Here we report the cytogenetic analysis of the THP-1 cell line using G-banding, fluorescence in situ hybridization (FISH), and spectral karyotyping (SKY), and the molecular characterization of the MLL-AF9 rearrangement by RT-PCR.
MLL-AF9对急性单核白血病细胞增殖的影响及其分子机制的初步研究
结果：从急性单核白血病细胞系thp-1克隆出了mll-af9断裂点附近序列，大小为528bp，测序后示mll基因断裂点位于外显子9，与af9基因外显子5发生融合。 结论：本实验成功克隆了THP-1细胞系MLL-AF9断裂点的 cDNA序列，为针对该序列设计特异性的siRNA进行基因沉默实验做 ...
抑制mll-af9融合基因表达对急性单核细胞白血病细胞THP-1增殖的 …
本研究探讨siRNA (small interfering RNA)对人急性单核细胞白血病细胞系THP-1的mll-af9融合基因的影响.选择THP-1细胞特有的m11-af9融合基因为靶基因,设计并合成 siRNA片段.以人急性单核细胞白血病细胞系THP-1为靶细胞,应用脂质体转染方法,将siRNA导入细胞,通过流式细胞仪检测siRNA转染效率, 用RTPCR法比较转染前后mll-af9 mRNA表达水平的变化,以MTT法检测细胞增生抑制率,流式细胞术检测细胞周期的改变和细胞凋亡水平.结果表明:siRNA转染效率为 …
RNAi-mediated silencing of MLL-AF9 reveals leukemia-associated ...
2014年2月11日 · In the AML cell line THP1 which harbors this t (9;11) translocation, endogenous MLL-AF9 was silenced via siRNA while ensuring specificity of the knockdown and its efficiency on functional protein level.
AURKA Suppresses Leukemic THP-1 Cell Differentiation through
2018年5月31日 · In this study, we focused on the regulation of the differentiation of the MLL-AF9 type AML cell line THP-1. We observed that, upon phorbol 12-myristate 13-acetate (PMA) treatment, THP-1 cells differentiated into monocytes by down-regulating Aurora kinase A (AURKA), resulting in a reduction in H3S10 phosphorylation.
MLL-AF9 oncogene expression affects cell growth but not terminal ...
2003年11月27日 · The MLL-AF9 oncogene - one of the most frequent MLL/HRX/ALL-1 rearrangements found in infantile and therapy-related leukaemias - originates from t(9;11)(p22;q23) and is mainly associated with monocytic acute myeloid leukaemia (AML-M5; FAB-classification). Here, we investigated the MLL-AF9 function b …
MLL-AF9对急性单核白血病细胞增殖的影响及其分子机制的初步研究
以人急性单核白血病细胞系THP-1为靶细胞,应用脂质体转染方法,将siRNA导入细胞,通过流式细胞仪检测siRNA转染效率,RT-PCR法比较转染前后MLL-AF9 mRNA表达水平的变化,MTT法检测细胞增生抑制率,并通过Hoechst33258染色观察siRNA作用于THP-1后细胞凋亡形态特征,流式细胞仪检 …