2011年3月31日 · Here we identify p53 target canonical and noncanonical REs in the family of Toll-like Receptor (TLR) innate immune response genes and establish p53 regulation of most TLR genes. We address p53 responsiveness in primary human lymphocytes and alveolar macrophages collected from healthy volunteers.

Reverse transcription PCR (RT-PCR) Polymerase chain reactions (PCR) were performed in the same reaction properties for toll like receptor 2, 4, CD14 and GAPDH according to the standard protocols with the primers indicated in Table 1. Briefly, cDNA (200 ng) was reacted with 250 mM dNTPs, 1 x reaction buffer (Fermentas), forward and reverse ...

2002年5月1日 · Results of RT-PCR on serial dilutions of eosinophil cDNA were compared with RT-PCR of serial dilutions of contaminating cell cDNAs and correlated with the cytospin counts. We found that purified eosinophils expressed mRNA for TLR4 in three of three samples thus analyzed and expressed MD-2 and CD14 in two of three samples.

Toll-like receptors (TLR) are key players of the innate and adaptive immune response in vertebrates. The original protein Toll in Drosophila melanogaster regulates both host defense and morphogenesis during development. Making use of real-time PCR, in situ hybridization, and immunohistochemistry we …

Background: Toll-like receptors (TLRs) are key factors in the innate immune system and initiate the inflammatory response to foreign pathogens such as bacteria, fungi...

2002年5月1日 · The Toll-like receptor (TLR)9 is critical for the recognition of immunostimulatory CpG motifs but may cooperate with other TLRs. We analyzed TLR1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of human PBMC and determined the sensitivity of these subsets to CpG oligodeoxynucleotides (ODN).

2004年2月15日 · In this work, we report novel real-time PCR methods for genotyping five TLR SNPs within TLR-2, TLR-4 and TLR-9 that have been associated with various diseases using fluorescence labeled hybridization probes and the LightCycler instrument.

2004年2月15日 · In this work, we report novel real-time PCR methods for genotyping five TLR SNPs within TLR-2, TLR-4 and TLR-9 that have been associated with various diseases using fluorescence labeled hybridization probes and the LightCycler™ instrument.

Toll-like receptors (TLRs) are members of the pattern recognition receptor family and mediate innate and adaptive immune responses. In this study, we isolated,...

2010年1月5日 · Surface and intracellular expression of TLRs was assessed by fluorescence-activated cell sorting and qRT-PCR. Cells were stimulated with TLR-specific ligands and cytokine production assessed by ELISA and cytokine bead array.