Recent data point to constitutive binding of SYK to the cytoplasmic domain of toll-like receptor-4 (TLR4). This SYK-TLR4 binding increases upon TLR4 dimerization and phosphorylation, and SYK plays a prominent role in TLR4 signalling in response to LPS in neutrophils and monocytes.

2013年8月20日 · We found that the nonreceptor tyrosine kinase Syk partially mediated the endocytosis of TLR4, but it also played a dual role in TLR4-mediated signaling. LPS-dependent stimulation of TLR4 in Syk-deficient macrophages led to enhanced activation of TAK1 and increased production of proinflammatory cytokines compared to that in wild-type macrophages.

2022年12月30日 · In this study, for the first time, in vivo, TLR4 and SYK were colocalized and had a high correlation in mastitis mammary epithelium; protein−protein interaction results also confirmed that there was a direct interaction between them in mastitis tissue, suggesting that SYK participates in the immune regulation of the TLR4 cascade for bovine ...

The function of Syk in the regulation of TLR4-mediated production of IFNβ and inflammatory cytokines is controversial. Here, we demonstrate that deficiency of Syk enhances the production of TLR4-mediated IFNβ and IL-10, and concurrently suppresses pro-inflammatory cytokine levels including TNFα and IL-6 in LPS-stimulated dendritic cells.

2022年12月30日 · To investigate the correlation of SYK and TLR4 in the innate immunity response of bMECs, this study focuses on the differential expression of SYK and TLR4 before or after LPS stimulation or S. agalactiae (GBS) in bovine MECs via siRNA, qRT-PCR, Western blot (WB) and immunofluorescence.

在这里，我们显示 scimp 招募 syk 以响应脂多糖介导的 tlr4 激活。 我们还表明 Syk 有助于 SCIMP 和 TLR4 的磷酸化以增强它们的结合。 进一步的证据表明 SCIMP 中的两个特定磷酸化位点对于在没有基于免疫受体酪氨酸的激活基序的情况下与 Syk-SH2 结构域的相互作用至关 ...

Syk deficiency resulted in decreased phosphorylation of IKKβ and the NF-κB p65 subunit, further suggesting a divergent influence of Syk on pro- and anti-inflammatory TLR responses. Conclusions: Syk negatively regulates TLR4-mediated production of IFNβ and IL-10 and promotes inflammatory responses in dendritic cells through divergent ...

2019年10月10日 · SYK constitutively binds to the cytoplasmic Toll/interleukin-1 receptor domain of TLR4 26 and plays a prominent role in TLR4 signaling in response to minimally modified LDL. 26,40 Depletion of SYK in J774 cells was achieved using siRNA treatment, and SYK protein levels were reduced by 80% compared with control siRNA-treated cells (Figure 4A and ...

2025年3月18日 · The LPS-TLR4 immune response is a critical mechanism in the body's defense against Gram-negative bacterial infections, yet its dysregulation can lead to severe inflammatory diseases. Lipopolysaccharide (LPS), a pivotal pathogen-associated molecular pattern (PAMP) on the surface of gram-negative bacteria, is recognized by Toll-like receptor 4 (TLR4), initiating a complex cascade of immune ...

Recent data point to constitutive binding of SYK to the cytoplasmic domain of toll-like receptor-4 (TLR4). This SYK-TLR4 binding increases upon TLR4 dimerization and phosphorylation, and SYK plays a prominent role in TLR4 signalling in response to LPS in neutrophils and monocytes.