In mice, Il33 deficiency severely attenuates inflammation- and LTβR-activation-induced TLSs in models of colitis and pancreatic ductal adenocarcinoma (PDAC). In PDAC, the alarmin domain of IL-33 activates group 2 innate lymphoid cells (ILC2s) expressing LT that engage putative LTβR+myeloid organizer cells to initiate tertiary lymphoneogenesis.

2025年3月10日 · Our study provides a comprehensive analysis of the expression profile of TA-TLS genes in PDAC (pancreatic ductal adenocarcinoma) and integrates TLS scoring and risk prognostic models for multidimensional bioinformatics analysis, filling the gap in the understanding of TLS gene characteristics and the tumor microenvironment in PDAC.

2021年3月17日 · The presence of tertiary lymphoid structures (TLS) has recently been shown to be predictive of checkpoint blockade response in melanomas and sarcomas, and are prognostic for survival in PDAC. In order to more comprehensively understand tumor immunity in PDAC patients with TLS, we performed RNA-seq, single and multiplex IHC, flow cytometry and ...

Previous publications propose a timeline for TLS development in which E-TLS precede M-TLS. 12,13 TLS can develop in tumors and correlate with improved disease-free and overall survival in many cancers, including PDAC. 14–16 The presence of TLS predicts ICB response in melanoma, sarcoma, and urothelial cancer, and ICB treatment can increase ...

2024年10月31日 · 最近研究表明，三级淋巴结构（TLS）的存在可预测黑色素瘤和肉瘤的检查点阻断反应，并可预测PDAC患者的生存。 为了更全面地了解PDAC患者TLS的肿瘤免疫，我们对治疗naïve、PDAC手术标本进行多组学分析。 46%的肿瘤含有不同的T细胞和B细胞聚集，反映了ES-TLS，这与更长的总生存期和无进展生存期相关。 这些肿瘤具有更大的CD8+ T细胞浸润，但未通过先前发表的TLS基因表达特征来定义。 ES-TLS+肿瘤富含IgG1类转换记忆B细胞和记 …

2024年7月1日 · Our literature review summarises the characteristics of tumour-associated tertiary lymphoid structures (TLSs) and highlight the key studies exploring the clinical outcomes of TLSs in PDAC patients and the direct effect on the TME.

2024年6月8日 · Tertiary lymphoid structures (TLSs) and CD8 + T-cells are potential prognostic indicators for pancreatic ductal adenocarcinoma (PDAC). We established a novel scoring system for evaluating the risk for PDAC based on TLS- and CD8 + T-cell-related genes. We analyzed single-cell sequence data from PDAC patients in the Genome Sequence Archive.

Consistent with this prediction, TRIP13 −/− PDAC cells were sensitive to the TLS inhibitor JH-RE-06 (Fig. 6A). TRIP13 deficiency also sensitized PDAC cells to gemcitabine (a first-line therapy for pancreatic cancer) and the WEE1 inhibitor AZD1775 (which has been explored as a potential treatment for PDAC) (Fig. 6A).

2025年3月17日 · In PDAC, fibroinflammation manifests in two discernible yet interdependent histological entities 209 — an immune-cold TME, ... CAFs regulate tertiary lymphoid structure (TLS) formation and ...

2024年12月11日 · This study elucidates shared and TLS/SLO-specific features in pancreatic ductal adenocarcinoma (PDAC). TLS abundance was related to superior survival and T-cell abundance in 110 treatment-naïve PDAC samples, underlining their clinical relevance. Immunofluorescence microscopy identified structural homologies between TLS and SLO.