分析后生动物 ATF6 在拟南芥中的同源基因，发现拟南芥 bZIP 家族的转录因子中，有 75 个成员具有类似于 ATF6 的特征，预测为二类跨膜蛋白。 其中 bZIP17/28/49 三个成员与之十分相似，并且它们的 C 端也具有 S1P（高尔基体腔内的一个蛋白酶）的切割位点。 内质网胁迫发生时，bZIP17/28 亚细胞定位转移到高尔基体。 在那里，可以被 S1P 蛋白酶切去膜内区域，被 S2P（高尔基体膜上的金属蛋白酶）切割跨膜结构，从而释放 bZIP 转录因子结构域进入细胞 …

研究人员采用原代小鼠肠上皮单层培养系统同时采用衣霉素（tunicamycin，Tm）处理进行scRNA-seq（衣霉素可以通过抑制蛋白质N-糖基化来诱导内质网应激）实验，该系统部分再现了极化上皮细胞的细胞组成，包括分泌杯状细胞和吸收肠细胞。

2018年1月5日 · Our study unravels the first ATF6-regulated gene expression network related to homeostatic regulation of membrane organelles, and provides novel mechanistic insights into aging-associated ...

2018年8月7日 · Pharmacologic arm-selective unfolded protein response (UPR) signaling pathway activation is emerging as a promising strategy to ameliorate imbalances in endoplasmic reticulum (ER) proteostasis implicated in diverse diseases.

2019年1月14日 · Here we show, using a mouse model of myocardial ischemia/reperfusion, that selective pharmacologic activation of the ATF6 arm of the unfolded protein response (UPR) during reperfusion, a typical...

2017年10月13日 · We recently isolated ATF6 as a candidate for mammalian UPR-specific transcription factor. We report here that ATF6 constitutively expressed as a 90-kDa protein (p90ATF6) is directly converted to a 50-kDa protein (p50ATF6) in ER-stressed cells.

Here we show that ATF6 decreases the activation of cardiac fibroblasts in response to the cytokine, transforming growth factor β (TGFβ), which can induce fibroblast trans-differentiation into a myofibroblast phenotype through signaling via the TGFβ–Smad pathway.

2007年9月4日 · ATF6α is neither essential for basal expression of ER protein chaperones nor for embryonic or postnatal development. However, ATF6α is required in both cells and tissues to optimize protein folding, secretion, and degradation during ER stress and thus to facilitate recovery from acute stress and tolerance to chronic stress.

该基因编码的转录因子可在内质网 (ER) 应激期间激活未折叠蛋白反应 (UPR) 的靶基因。 虽然它是一种转录因子，但这种蛋白质的不同寻常之处在于它是作为嵌入 ER 中的跨膜蛋白合成的。 它作为 ER 应激传感器/转换器发挥作用，在 ER 应激诱导的蛋白水解后，它通过存在于编码 ER 伴侣蛋白的基因启动子中的顺式作用 ER 应激反应元件 (ERSE) 作为核转录因子发挥作用。 这种蛋白质已被确定为静止而非增殖性鳞状癌细胞的存活因子。 关于该基因多态性与不同人群糖尿病相关性 …

2000年9月1日 · ATF6 bound the ATF6m1 site significantly more weakly than the ATF6 site (Fig. 3 B, lanes 1 and2), suggesting that the G flanking the TGACGT core is critical to ATF6-specific binding. In contrast, we found that purified ATF1 bound all three sites, with stronger binding to the CRE and ATF6m1 sites (Fig. 3 B , lanes 4–6 ).