3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). [1] . TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate.

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species ( ROS/RNS ), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to ...

The rate constant for compound II reduction to native enzyme by TMB obtained under steady-state conditions is (9.4 +/- 0.6) x 10(5) M-1 s-1. The results are applied to a new, more accurate assay for myeloperoxidase based upon the formation of the charge-transfer complex between TMB and its diimine final product.

tmb已在逐步取代强致癌物联苯胺和其他致癌性的联苯胺衍生物，应用于临床化验、法医检验、刑事侦破及环境监测等领域；尤其是在临床生化检验方面，tmb作为过氧化酶的新底物，在酶免疫分析法（eia）和酶联免疫吸附检验法（elisa）中获得了广泛的应用。

3 天之前 · TMB (3,3', 5,5"-tetramethylbenzidine) is used as a substrate to generate detectable signal in ELISA. The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color change that correlates with analyte level.

2020年11月4日 · 自从3,3',5,5'-四甲基联苯胺（tmb）首次在基于hrp的免疫分析中应用以来，tmb是迄今为止最常用的生色底物，其显色反应基于h2o2存在下hrp催化氧化tmb。 在TMB出现之后，逐渐取代了其他联苯胺衍生物在比色测定中的使用。

3,3,5,5,Tetramethylbenzidine (TMB) is a colorimetric substrate used with peroxidase and peroxidase-coupled systems. It produces a soluble end product, which is blue in color and can be read spectrophotometrically at 370 nm or 655 nm( photometric mode).

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and …

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce ...