2011年11月17日 · We report here that RIP1 is a crucial mediator of canonical NF- κ B activation in L929 cells, therefore questioning the relative cytoprotective contribution of RIP1 ubiquitination versus...

Our results suggest that L929 cells treated with TNF-alpha alone show nuclear changes and a pattern of DNA fragmentation that are atypical of apoptosis. In contrast, our results demonstrate that, when augmented with actinomycin D, TNF-alpha induces classical apoptosis in L929 cells.

2015年1月23日 · To explore this issue, we introduced supernatants from L929 cells treated with TNF alone or TNF/zVAD (necroptotic) into the peritoneal cavities of immunocompetent BALB/c mice and monitored...

1997年2月1日 · The role of protein kinase C (PKC) in tumour necrosis factor (TNF)-mediated cytotoxicity was investigated using the L929 cell line. The PKC activator phorbol-12-myristate 13-acetate (PMA) increased proliferation and inhibited TNF-induced cytotoxicity of L929 cells.

1999年10月1日 · Our results suggest that L929 cells treated with TNF-α alone show nuclear changes and a pattern of DNA fragmentation that are atypical of apoptosis. In contrast, our results demonstrate that, when augmented with actinomycin D, TNF-α …

It has been shown that necroptosis—caspase-independent programmed necrotic cell death—can be induced by treatment with tumor necrosis factor (TNF) in the L929 murine fibrosarcoma cell line, even in the absence of a caspase inhibitor.

Treatment of L929 cells with tumour necrosis factor-alpha (TNF-alpha) plus actinomycin D induced DNA damage (indicated by the appearance of a sub-G1 peak due to extracellular leakage of low molecular weight DNA following DNA …

TNF 受体 1 信号在 L929 细胞中诱导 NF-κB 活化和坏死性凋亡。 我们之前报道了细胞凋亡蛋白介导的受体相互作用蛋白 1 (RIP1) 泛素化抑制剂作为细胞保护机制，而圆柱瘤病是一种 RIP1 去泛素化酶，可防止肿瘤坏死因子 (TNF) 诱导的坏死性凋亡。 我们在此报告 RIP1 是 L929 细胞中经典 NF-κB 激活的关键介质，因此质疑 RIP1 泛素化与经典 NF-κB 激活的相对细胞保护作用。 我们发现减弱的 NF-κB 激活对 TNF 诱导的坏死性凋亡没有影响。 然而，我们发现 A20 和线性泛素 …

最近的研究不同程度地报道了肿瘤坏死因子 α (TNF-α) 在 L929 细胞中诱导坏死或凋亡。 进行这项研究是为了更好地表征该药剂在 L929 细胞中诱导的死亡模式。 我们确定了暴露于 TNF-α 和其他细胞毒性剂对细胞大小和形态、细胞膜通透性、细胞表面磷脂酰丝氨酸的暴露、核形态和 DNA 片段化的影响。 我们的结果表明，单独用 TNF-α 处理的 L929 细胞显示出细胞核变化和非典型细胞凋亡的 DNA 片段化模式。 相比之下，我们的结果表明，当用放线菌素 D 增强时，TNF-α 在 L929 …

This RNAi study in L929 cells demonstrates how knockdown of several TNF-signaling proteins involved in TNFR1-CI and II strongly modulate TNF-induced necroptosis .