Ionization and structural properties of mRNA lipid nanoparticles ...
2021年8月11日 · By performing the TNS binding assay at a constant ionizable lipid concentration (accounting for the NP lipid:mRNA ratio), we observed that lower LNPs with lower NP …
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真正的创新，犹如璀璨星辰，不仅在于技术领域的惊天一跃，更在于如何将这璀璨光芒转化为推动生命科学进步的不竭动力。 因此，我们提供全方位的技术支持、定制化解决方案以及持续的 …
TNS Assay: Detailed Insights for Protein and Lipid Research
2025年3月13日 · The TNS assay is a powerful tool for tracking protein conformational changes, particularly transitions between folded and unfolded states. When proteins undergo structural …
2.5. Evaluation of Surface pKa by TNS Assay - Bio-protocol
For the 6-(p-Toluidino)-2-naphthalenesulfonyl chloride (TNS) assay, 20 mM citric acid/NaOH buffer (with 150 mM NaCl, pH 3.0, 3.5, 4.0, 4.5, 5.0, 5.5), 20 mM sodium dihydrogen...
The Importance of Apparent pKa in the Development of …
Acid-base titration and 2-(p-toluidino)-6-naphthalene sulfonic acid (TNS) fluorescent methods are widely used in the determination of apparent pKa of blank nanoparticles. In the acid-base …
LipidLaunch™ LNP表观pKa测定试剂盒测定脂质纳米粒的表观pKa
2025年3月18日 · TNS在解离时会失去荧光，因此在LNP存在下滴定pH会产生一条曲线，其中50%最大荧光点称为颗粒的表观pKa。 Cayman Chemical LipidLaunch™ LNP表观pKa测定试 …
acid sodium salt (TNS). This probe fluoresces in association with positively charged membranes, which can be easily quantified using a fluorescence plate reader at excitation and emission …
tns荧光法测定pka的原理 - 百度文库
TNS荧光法测定pKa的原理是基于滴定曲线和等值点的概念。在滴定曲线中，有两个等值点，这些等值点对应的pH值即为pKa值。TNS荧光法非常灵敏，已被广泛用于测量LNPs的pKa。
Mixing lipids to manipulate the ionization status of lipid ...
2018年9月18日 · The pKa values for the YSK05/12-LNP membranes were determined using a TNS assay. TNS is a compound that electrostatically interacts with the cationic lipid …
LipidLaunch™ LNP Apparent pKa Assay Kit (TNS Method)
Cayman’s LipidLaunch™ LNP Apparent pK a Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pK a of formulated LNPs. The assay uses …