2021年9月11日 · Here, we show that tripartite motif protein TRIM21 acts as a positive regulator of GSDMD-dependent pyroptosis. TRIM21 interacted with GSDMD via its PRY-SPRY domain, maintaining GSDMD...

2021年9月14日 · 这项研究揭示了trim21在gsdmd介导的焦亡中发挥重要作用，为控制和治疗炎症相关疾病提供了全新作用靶标。 图示：trim21促进gsdmd高聚，从而正向调控细胞焦亡。 高文青博士和博士研究生李媛媛为论文共同第一作者，李继喜教授和陈向军教授为论文共同通讯作者。

TRIM21 interacted with GSDMD via its PRY-SPRY domain, maintaining GSDMD stable expression in resting cells yet inducing the N-terminus of GSDMD (GSDMD-N) aggregation during pyroptosis. TRIM21-deficient cells displayed a reduced cell death in response to NLRP3 or NLRC4 inflammasome activation.

SCU degrades p30/GSDMD through ubiquitin-dependent autophagic machanism. E3 ligase TRIM21 plays an important role in the degradation induced by SCU. Most of the pyroptosis inhibitors targeted Gasdermin D (GSDMD) are functioning by …

2021年9月13日 · 这项研究揭示了 trim21 在 gsdmd 介导的焦亡中发挥重要作用，为控制和治疗炎症相关疾病提供了全新作用靶标。 trim21 促进 gsdmd 高聚，从而正向调控细胞焦亡。 高文青博士和李媛媛博士研究生为论文共同第一作者，李继喜教授和陈向军教授为论文共同通讯作者。

2021年9月15日 · 文章揭示， PRY-SPRY结构域是TRIM21结合GSDMD的关键结构，并维持细胞中GSDMD稳定表达，且在细胞焦亡过程中诱导GSDMD-N发生高聚。 NLRP3或NLRC4炎性小体激活后，相比于对照组，TRIM21缺陷型细胞死亡率显著降低；同时，在沙门氏菌感染情况下，TRIM21敲除的BMDM细胞存活率也显著增加。 此外，TRIM21敲除的小鼠在脂多糖（LPS）诱导的败血症和葡聚糖硫酸钠（DSS）诱导的结肠炎模型中，出现的炎症反应明显减弱，死亡率明 …

Here, we show that tripartite motif protein TRIM21 acts as a positive regulator of GSDMD-dependent Pyroptosis. TRIM21 interacted with GSDMD via its PRY-SPRY domain, maintaining GSDMD stable expression in resting cells yet inducing the N-terminus of GSDMD (GSDMD-N) aggregation during Pyroptosis .

TRIM21 directly interacts with GSDMD through the PRY-SPRY domain in vivo and in vitro to maintain the stable expression of GSDMD in cell . The discovery of this regulatory factor also provides a new target for fine-tuning and treatment of inflammation-related diseases.

Here, we show that tripartite motif protein TRIM21 acts as a positive regulator of GSDMD-dependent pyroptosis. TRIM21 interacted with GSDMD via its PRY-SPRY domain, maintaining GSDMD...

2023年11月1日 · Gasdermin-D (GSDMD) is crucial for releasing inflammatory cytokines during pyroptosis. In this pathway, caspase-1 is activated within the inflammasome, cleaving gasdermin-D (GSDMD) into two fractions: GSDMD-N and GSDMD-C. TRIM21 can interact (by PRYSPRY domain) with GSDMD-N, making a complex leading to GSDMD-N oligomerization.