Trimethylsilylpropanoic acid (TMSP or TSP) is a chemical compound containing a trimethylsilyl group. It is used as internal reference in nuclear magnetic resonance for aqueous solvents (e.g. D 2 O). For that use it is often deuterated (3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid …

The study showcases a novel method that combines pseudo two-dimensional liquid flash chromatography with NMR spectroscopy, providing a faster, more accurate analytical approach. This method significantly enhances the efficiency and reliability of chemical analysis in pharmaceutical and biomedical research, making it a valuable technique for ...

Trimethylsilylpropanoic acid (TMSP or TSP) is a chemical compound containing a trimethylsilyl group. It is used as internal reference in nuclear magnetic resonance for aqueous solvents (e.g. D2O). For that use it is often deuterated (3- (trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or …

2023年6月16日 · Deuterated 3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid (TSP) is used as a chemical shift internal reference in 1 H-NMR analysis of aqueous solvents. The TSP peak can also be used for relative quantitation to correct for sample loss during preparation and/or as a reference peak to calculate absolute concentration of metabolites since a ...

2016年1月1日 · Two technologies are routinely used in order to analyse metabolite profiles in biological fluids: nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), the latter typically with hyphenation to a chromatography system such as liquid chromatography (LC), in a configuration known as LC–MS.

2024年9月12日 · Following the published protocol, we here use sodium trimethylsilylpropanesulfonate (DSS, (CH₃)₃SiCH₂CH₂CH₂SO₃⁻Na⁺) or trimethylsilylpropanoic acid (TMSP or TSP) to collect 1 H spectra at different temperatures to obtain the “shifted” values of DSS (or TSP) at 0.0 ppm.

2017年8月8日 · We recommend a concentration of about 0.02 mM of TSP or DSS in the NMR samples of intracellular metabolites and about 0.2–0.4 mM for culture medium samples. These concentrations ensure that the internal standard will have a resonance with sufficient S/N for accurate quantitation and at the same time an intensity within the average dynamic ...

3- (trimethylsilyl)propionic acid is an organosilicon compound that is propionic acid substituted at position 3 by a trimethylsilyl group. It is used as internal reference in the NMR spectrum nuclear magnetic resonance for aqueous solvents (e.g. D2O). It is an organosilicon compound and a monocarboxylic acid.

2010年2月25日 · TSP is a reference for the calibration of NMR shifts, and can also serve as a reference (internal standard) in the quantitation of metabolites.

Journal of Biomolecular NMR - A marked dependence of the 1H resonances of TSP and DSS (internal standards) on the concentration of proteins in the molten globule state has been found. This result...